Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP18343] to BS69 - ChIP Grade
  • Suitable for: WB, ChIP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-BS69 antibody [EP18343] - ChIP Grade
    See all BS69 primary antibodies

  • Description

    Rabbit monoclonal [EP18343] to BS69 - ChIP Grade

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ChIP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal brain and fetal kidney lysates; HeLa, 293T, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; mouse brain, kidney and spleen lysates; rat brain, kidney and spleen lysates; ChIP: Chromatin prepared from HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • ChIP - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    ChIP - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)

    Chromatin was prepared from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab190890 (blue), and 20µl of Anti rabbit IgG sepharose beads. 2μg of rabbit normal IgG was added to the beads control (yellow).  The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).

  • Western blot - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    Western blot - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    All lanes : Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890) at 1/1000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    Western blot - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    All lanes : Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890) at 1/1000 dilution

    Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    Western blot - Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    All lanes : Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat kidney lysate
    Lane 6 : Rat spleen lysate
    Lane 7 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 8 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 10 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1, 2, 3, 4, 5 and 6: 30 seconds; Lane 7, 8, 9 and 10: 15 seconds.

  • Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)
    Anti-BS69 antibody [EP18343] - ChIP Grade (ab190890)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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