Antibodies, Proteins, Kits and Reagents for Life Science

375 244 ₸ Product size

1 x 96 tests 375 244 ₸

Order now and get it on Thursday February 25, 2021

Human MIP1 beta ELISA Kit (CCL4) (ab100597)

Key features and details

Sensitivity: 2.5 pg/ml
Range: 4.1 pg/ml - 1000 pg/ml
Sample type: Cell culture supernatant, Plasma, Serum
Detection method: Colorimetric
Assay type: Sandwich (quantitative)
Reacts with: Human

Storage instructions

Store at -20°C. Please refer to protocols.

Components	1 x 96 tests
20X Wash Buffer Concentrate	1 x 25ml
5X Assay Diluent B	1 x 15ml
600X HRP-Streptavidin Concentrate	1 x 200µl
Assay Diluent A	1 x 30ml
Biotinylated anti-Human MIP1 beta	2 vials
MIP1 beta Microplate (12 x 8 wells)	1 unit
Recombinant Human MIP1 beta Standard (lyophilized)	2 vials
Stop Solution	1 x 8ml
TMB One-Step Substrate Reagent	1 x 12ml

Research areas
Function

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.
Post-translational
modifications

N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
Cellular localization

Secreted.
Target information above from: UniProt accession P13236 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Alternative names
- MIP 1 beta
- Secreted protein G 26
- ACT 2
- ACT-2
- ACT2
- AT744.1
- AT744.2
- C C motif chemokine 4
- C C motif chemokine 4 like
- C C motif chemokine ligand 4 like 1
- C C motif chemokine ligand 4 like 2
- CC chemokine ligand 4
- CC chemokine ligand 4L1
- CC chemokine ligand 4L1d2
- CC chemokine ligand 4L2
- CCL4
- CCL4_HUMAN
- CCL4L
- ccl4l 1
- CCL4L1
- Chemokine (C C motif) ligand 4
- Chemokine (C C motif) ligand 4 like 1
- Chemokine (C C motif) ligand 4 like 1, telomeric
- Chemokine (C C motif) ligand 4 like 2
- Chemokine CC Motif Ligand 4
- G 26
- G 26 T lymphocyte secreted protein
- G-26 T-lymphocyte-secreted protein
- HC21
- Immune activation 2
- LAG 1
- LAG-1
- LAG1
- Lymphocyte activation gene 1
- Lymphocyte activation gene 1 protein
- Macrophage inflammatory protein 1 beta
- Macrophage inflammatory protein 1-beta
- Macrophage inflammatory protein 1b2
- MGC104418
- MGC126025
- MGC126026
- MIP-1-beta
- MIP-1-beta(1-69)
- MIP-1-beta(3-69)
- MIP1 beta
- MIP1B
- MIP1B1
- Monocyte adherence induced protein 5 alpha
- PAT 744
- Protein H400
- SCYA2
- SCYA4
- SCYA4L
- SCYA4L1
- SCYA4L2
- SCYQ4L2
- Secreted protein G 26
- Secreted protein G26
- SIS gamma
- SIS-gamma
- Small inducible cytokine A4
- Small inducible cytokine A4 (homologous to mouse Mip 1b)
- small inducible cytokine A4-like
- Small-inducible cytokine A4
- T cell activation protein 2
- T-cell activation protein 2
see all
Database links
- Entrez Gene: 388372 Human
- Entrez Gene: 6351 Human
- Entrez Gene: 9560 Human
- Omim: 182284 Human
- Omim: 610757 Human
- Omim: 603782 Human
- SwissProt: Q8NHW4 Human
- SwissProt: P13236 Human
- Unigene: 655293 Human
- Unigene: 661942 Human
- Unigene: 75703 Human
see all

Typical Standard Curve

Representative Standard Curve using ab100597.
Typical Standard Curve

Representative Standard Curve using ab100597.

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