Human PSG1 ELISA Kit (ab243676)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 11.5 pg/ml
- Range: 25 pg/ml - 1600 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PSG1 ELISA Kit
See all PSG1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 5.6% Inter-assay Sample n Mean SD CV% serum 3 1.4% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
11.5 pg/ml -
Range
25 pg/ml - 1600 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 97 88% - 106% Urine 88 84% - 90% Serum 101 94% - 115% Cell culture media 93 89% - 98% Hep Plasma 88 76% - 94% EDTA Plasma 88 82% - 94% Cit plasma 88 77% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human PSG1 ELISA Kit (ab243676) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSG1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PSG1 with 11.5 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human PSG1 Capture Antibody 1 x 600µl 10X Human PSG1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human PSG1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 2 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Sequence similarities
Belongs to the immunoglobulin superfamily. CEA family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Developmental stage
PSBG are produced in high quantity during pregnancy. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- B1G1
- Carcinoembryonic antigen SG8
- CD66 antigen-like family member F
see all -
Database links
- Entrez Gene: 5669 Human
- Omim: 176390 Human
- SwissProt: P11464 Human
- Unigene: 709192 Human
Images
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Other - Human PSG1 ELISA Kit (ab243676)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human PSG1 Standard Curve Comparison.Standard Curve comparison between human PSG1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of human PSG1 standard curve in Sample Diluent NS.The PSG1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native PSG1 in pregnant human serum and SW480 cell culture supernatant samples.The concentrations of PSG1 were measured in duplicates, interpolated from the PSG1 standard curves and corrected for sample dilution. Undiluted samples are as follows: pregnant human serum 1:20,000 and SW480 cell culture supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSG1 concentration was determined to be 18.13 µg/mL in neat pregnant human serum and 0.0049 µg/mL in neat SW480 cell culture supernatant.
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Sandwich ELISA - Human PSG1 ELISA Kit (ab243676)To learn more about the advantages of recombinant antibodies see here.
