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Human REG3A ELISA Kit (ab267626)

Key features and details

  • Sensitivity: 0.12 ng/ml
  • Range: 0.12 ng/ml - 25 ng/ml
  • Sample type: Cell culture supernatant, Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human REG3A ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall
    Inter-assay
    Sample n Mean SD CV%
    Overall

  • Sample type

    Cell culture supernatant, Serum, Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.12 ng/ml

  • Range

    0.12 ng/ml - 25 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 128.5 117% - 138%
    Plasma 119.1 97% - 146%
    Cell culture media 121.5 109% - 140%

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human REG3A ELISA Kit is designed for the quantitative determination of REG3A in cell culture supernatants, plasma and serum samples.


    This assay employs an antibody specific for human REG3A coated on a 96-well plate. Standards and samples are pipetted into the wells and REG3A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human REG3A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of REG3A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    This ELISA kit shows no cross-reactivity with the following cytokines tested: Human ADAM22, Arylsulfatase B/ARSB, B3GNT2, Carbonic Anhydrase VB, Caspase 7, Caspase 8, Integrin alpha M/CD11b, SIRP gamma/CD172g, CD39L2/ENTPD6, CD39L4/ENTPD5, Integrin alpha 2/CD49b, CD7, CEACAM3, Carboxypeptidase E/CPE, FABP6, FAM3C, GDF-3, GSTM1, Kallikrein 11, Kallikrein 12, Kremen-2, OSCAR, PTP1B, R-Spondin 2, S100A13, Semaphorin 4C, Sirtuin 1/SIRT1, SMPD1, Sortilin, SPINK1, Stabilin-2, SULT2A1, Transcobalamin II, THSD1, TrkA, UCH-L3, VAPA, vWF-A2, Wnt-4.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    5X Assay Diluent D 1 x 15ml
    Anti-Human REG3A coated Microplate (12 x 8 wells) 1 unit
    Biotinylated Anti-Human REG3A Detection Antibody 2 vials
    Human REG3A Standard (Lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB Substrate Solution 1 x 12ml

  • Research areas

  • Function

    Bactericidal C-type lectin which acts exclusively against Gram-positive bacteria and mediates bacterial killing by binding to surface-exposed carbohydrate moieties of peptidoglycan. Regulates keratinocyte proliferation and differentiation after skin injury via activation of EXTL3-PI3K-AKT signaling pathway.

  • Tissue specificity

    Highly expressed in epidermal keratinocytes of psoriasis patients (at protein level). Constitutively expressed in intestine. Low expression is found in healthy pancreas. Overexpressed during the acute phase of pancreatitis and in some patients with chronic pancreatitis.

  • Sequence similarities

    Contains 1 C-type lectin domain.

  • Domain

    The EPN motif is essential for recognition of the peptidoglycan carbohydrate backbone and for efficient bacterial killing with Glu-114 playing a key role in peptidoglycan binding and bactericidal activity.

  • Post-translational
    modifications

    Proteolytic processing by trypsin removes an inhibitory N-terminal propeptide and is essential for peptidoglycan binding and antibacterial activity.

  • Cellular localization

    Secreted. Found in the apical region of pancreatic acinar cells.
  • Information by UniProt

  • Alternative names

    • FLJ18565
    • Hepatocarcinoma intestine pancreas
    • Hepatointestinal pancreatic protein
    • HIP
    • HIP/PAP
    • Human proislet peptide
    • INGAP
    • Pancreatic beta cell growth factor
    • Pancreatitis associated protein
    • Pancreatitis associated protein 1
    • Pancreatitis-associated protein 1
    • PAP
    • PAP H
    • PAP homologous protein
    • PAP1
    • PBCGF
    • Proliferation inducing protein 34
    • Proliferation inducing protein 42
    • REG III
    • Reg III alpha
    • Reg III-alpha
    • REG-3-alpha
    • REG3
    • Reg3a
    • REG3A_HUMAN
    • Regenerating islet derived 3 alpha
    • Regenerating islet derived protein 3 alpha precursor
    • Regenerating islet-derived protein 3-alpha 15 kDa form
    • Regenerating islet-derived protein III-alpha
    see all

  • Database links

    Images

    • Example data
      Example data

      This standard curve is for demonstration only. A standard curve must be run with each assay.

       

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