6 Phosphogluconate Assay Kit (Colorimetric) (ab211071)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 20 µM
Overview
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Product name
6 Phosphogluconate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
20 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
6-Phosphogluconate Assay Kit (Colorimetric) (ab211071) provides a convenient method to detect 6-phosphogluconate (6-PGA) in cell and tissue lysates. In this assay, 6-phosphogluconate is converted to ribulose-5-phosphate by 6-phosphogluconate dehydrogenase in the presence of NAD+. NADH formed in the reaction reduces the probe present in the reaction mix, generating a strong absorbance at OD 450 nm. This absorbance is proportional to the amount of 6-PGA present in the samples.This assay kit is fast, sensitive and high-through adaptable. It can measure less than 20 µM of 6-phosphogluconate present in the sample.
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Notes
6-phosphogluconate (6-phosphgluconic acid, 6-PGA) is an intermediate of both Pentose Phosphate Pathway (PPP) and Entner-Doudoroff Pathway. In the Pentose Phosphate Pathway, 6-PGA is converted by 6-phosphogluconate dehydrogenase into ribulose-5-phosphate, a precursor for the synthesis of nucleic acids. In prokaryotes, 6-PGA is the main metabolite of the Entner-Doudoroff pathway, and is converted into pyruvate by both 6-phosphogluconate dehydratase and 2-keto-3-Deoxyphosphogluconate aldolase.
Recent studies show that long-term exposure to glucose perturbs the PPP, causing significant accumulation of 6-PGA and impairment of beta cell function in the pancreas. Measurement of 6-PGA levels is important when evaluating therapies for diabetes research.
In pathological bacteria, measurement of 6-PGA levels and functionality of Entner-Doudoroff pathway can be used for clinical and diagnostic purposes.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests PGA Assay Buffer 1 x 25ml 6-PGA Enzyme (15 mg) 1 vial 6-PGA Standard (10 mg) 1 vial 6-PGA Substrate Mix (25 mg) 1 vial
Images
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6-Phosphogluconate Assay Kit (Colorimetric) (ab211071)
Typical 6-phosphogluconate (6-PGA) standard calibration curve.
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6-Phosphogluconate Assay Kit (ab211071)Measurement of 6-PGA in lysates from rat liver (160 µg), rat kidney (120 µg) and rat heart (60 µg).
