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Anti-ATPB antibody [EPR11990] (ab170947)

Price and availability

304 886 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-ATPB antibody [EPR11990] (ab170947)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11990] to ATPB
  • Suitable for: WB, IHC-P, ICC/IF, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-ATPB antibody [EPR11990]
    See all ATPB primary antibodies
  • Description

    Rabbit monoclonal [EPR11990] to ATPB
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human ATPB aa 150-250 (Cysteine residue). The exact sequence is proprietary.
    Database link: P06576

  • Positive control

    • HepG2; 293T; HeLa and HT-29 cell lysates; Human heart and liver tissues; HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11990
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex V

Images

  • Western blot - Anti-ATPB antibody [EPR11990] (ab170947)
    Western blot - Anti-ATPB antibody [EPR11990] (ab170947)
    All lanes : Anti-ATPB antibody [EPR11990] (ab170947) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : HT29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] (ab170947)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] (ab170947)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labeling ATPB with purified ab170947 at 1/500 dilution. Cells were fixed with 100% methanol. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)

    ab170947 showing +ve staining in Human normal kidney tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)

    ab170947 showing +ve staining in Human normal uterus tissue.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling ATPB with ab170947 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [EPR11990] (ab170947)

    Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling ATPB with ab170947 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] (ab170947)
    Immunocytochemistry/ Immunofluorescence - Anti-ATPB antibody [EPR11990] (ab170947)

    Immunofluorescent analysis of HeLa cells labeling ATPB using ab170947 at 1/50 dilution (green). DAPI nuclear staining (blue).

  • Immunoprecipitation - Anti-ATPB antibody [EPR11990] (ab170947)
    Immunoprecipitation - Anti-ATPB antibody [EPR11990] (ab170947)
    Anti-ATPB antibody [EPR11990] (ab170947) at 1/10 dilution + HepG2 cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
  • Anti-ATPB antibody [EPR11990] (ab170947)
    Anti-ATPB antibody [EPR11990] (ab170947)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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