Antibodies, Proteins, Kits and Reagents for Life Science

Key features and details

Mouse monoclonal [6C10] to ATPB
Suitable for: WB, IHC-P
Reacts with: Rat, Dog, Human, African green monkey
Isotype: IgG2b

Product name

Anti-ATPB antibody [6C10]
See all ATPB primary antibodies
Description

Mouse monoclonal [6C10] to ATPB
Host species

Mouse
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Recombinant full length protein corresponding to Human ATPB. NP_001677. Produced in HEK-293T cells.
Database link: P06576
Positive control
- IHC-P: Human skeletal muscle tissue. WB: pCMV6-ENTRY ATPB transfected HEK-293T cell lysate. HeLa, HepG2, MDCK and PC-12 cell extracts.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purity

Protein A/G purified
Clonality

Monoclonal
Clone number

6C10
Isotype

IgG2b
Research areas

Western blot - Anti-ATPB antibody [6C10] (ab230706)

All lanes : Anti-ATPB antibody [6C10] (ab230706) at 1/2000 dilution

Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell extracts
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell extracts
Lane 3 : HT-29 (Human colorectal adenocarcinoma cell line) cell extracts
Lane 4 : A549 (Human lung carcinoma cell line) cell extracts
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) cell extracts
Lane 6 : Jurkat (Human T cell leukemia cell line from peripheral blood) cell extracts
Lane 7 : MDCK (Canine kidney cell line) cell extracts
Lane 8 : PC-12 (Rat adrenal gland pheochromocytoma cell line) cell extracts
Lane 9 : MCF7 (Human breast adenocarcinoma cell line) cell extracts

Lysates/proteins at 35 µg per lane.

Developed using the ECL technique.

Predicted band size: 57 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATPB antibody [6C10] (ab230706)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for ATPB with ab230706 at a 1:50 dilution in immunohistochemical analysis.
Western blot - Anti-ATPB antibody [6C10] (ab230706)

All lanes : Anti-ATPB antibody [6C10] (ab230706) at 1/2000 dilution

Lane 1 : pCMV6-ENTRY control transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY ATPB transfected HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lysates/proteins at 5 µg per lane.

Developed using the ECL technique.

Predicted band size: 57 kDa

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