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Anti-RACGAP1/MGCRACGAP antibody (ab97315)

Anti-RACGAP1/MGCRACGAP antibody (ab97315)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to RACGAP1/MGCRACGAP
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-RACGAP1/MGCRACGAP antibody
    See all RACGAP1/MGCRACGAP primary antibodies
  • Description

    Rabbit polyclonal to RACGAP1/MGCRACGAP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human RACGAP1/MGCRACGAP aa 1-172. (AAH32754)

  • Positive control

    • WB: A431 and Raji whole cell, mouse heart tissue lysate; IHC-P: Human hepatoma and H1299 xenograft tissue; IF: A431 whole cell.
  • General notes

     This product was previously labelled as RACGAP1

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab97315)

    Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining RACGAP1 with ab97315 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

  • Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
    Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
    All lanes : Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 71 kDa



    7.5% SDS-PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
    Immunocytochemistry/ Immunofluorescence - Anti-RACGAP1/MGCRACGAP antibody (ab97315)

    Confocal immunofluorescence analysis of paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) whole cells labeling RACGAP1 (green) with ab97315 at 1/500 dilution. Alpha-tubulin filaments (red), at 1:2000 dilution.

     

  • Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
    Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
    Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution + Mouse heart tissue lysate at 50 µg

    Predicted band size: 71 kDa



    7.5% SDS PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab97315)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab97315)

    Immunohistochemical analysis of paraffin-embedded H1299 (human non-small cell lung carcinoma cell line) xenograft tissue staining RACGAP1 with ab97315 at 1/500 dilution.

    Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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