Anti-RACGAP1/MGCRACGAP antibody (ab97315)
Key features and details
- Rabbit polyclonal to RACGAP1/MGCRACGAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RACGAP1/MGCRACGAP antibody
See all RACGAP1/MGCRACGAP primary antibodies -
Description
Rabbit polyclonal to RACGAP1/MGCRACGAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human RACGAP1/MGCRACGAP aa 1-172. (AAH32754)
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Positive control
- WB: A431 and Raji whole cell, mouse heart tissue lysate; IHC-P: Human hepatoma and H1299 xenograft tissue; IF: A431 whole cell.
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General notes
This product was previously labelled as RACGAP1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded human hepatoma tissue staining RACGAP1 with ab97315 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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All lanes : Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 71 kDa
7.5% SDS-PAGE -
Confocal immunofluorescence analysis of paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) whole cells labeling RACGAP1 (green) with ab97315 at 1/500 dilution. Alpha-tubulin filaments (red), at 1:2000 dilution.
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Anti-RACGAP1/MGCRACGAP antibody (ab97315) at 1/1000 dilution + Mouse heart tissue lysate at 50 µg
Predicted band size: 71 kDa7.5% SDS PAGE
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Immunohistochemical analysis of paraffin-embedded H1299 (human non-small cell lung carcinoma cell line) xenograft tissue staining RACGAP1 with ab97315 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.