Anti-RACGAP1/MGCRACGAP antibody (ab2270)
Key features and details
- Goat polyclonal to RACGAP1/MGCRACGAP
- Suitable for: IHC-P, ICC, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RACGAP1/MGCRACGAP antibody
See all RACGAP1/MGCRACGAP primary antibodies -
Description
Goat polyclonal to RACGAP1/MGCRACGAP -
Host species
Goat -
Tested applications
Suitable for: IHC-P, ICC, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow, Dog
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Immunogen
Synthetic peptide corresponding to Human RACGAP1/MGCRACGAP aa 620-632 (C terminal).
Sequence:C-GRQGNFFASPMLK
(Peptide available asab22898) -
Positive control
- WB: Human testis tissue lysate. K562 and Jurkat cell lysates. ICC: MCF7 cells. IHC-P: Human testis tissue.
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General notes
Principal Names - RACGAP1/MGCRACGAP; Rac GTPase activating protein 1; ID-GAP; MgcRacGAP; GTPase activating protein. Official Gene Symbol - - RACGAP1/MGCRACGAP. GenBank Accession Number – NP_037409. LocusLink ID - 29127.
This product was previously labelled as RACGAP1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)All lanes :
Lane 1 : K562 cell lysate (in RIPA buffer)
Lane 2 : Jurkat cell lysate (in RIPA buffer)
Lysates/proteins at 35 µg per lane.
Predicted band size: 71 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RACGAP1/MGCRACGAP antibody (ab2270)ab2270 (1µg/ml) staining Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer (pH9), HRP-staining. -
Immunocytochemistry - Anti-RACGAP1/MGCRACGAP antibody (ab2270)Immunoctochemistry analysis of paraformaldehyde fixed MCF7 (human breast adenocarcinoma cell line) cells labeling RACGAP1/MGCRACGAP with ab2270 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation 1 hour followed by Alexa Fluor® 488 secondary antibody (4 µg/mL). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (4 µg/mL). -
Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 0.2 µg/ml + Human Testis lysate (RIPA buffer) at 30 µg
Predicted band size: 71 kDaab2270 staining (0.2µg/ml) of Human Testis lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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Western blot - Anti-RACGAP1/MGCRACGAP antibody (ab2270)Anti-RACGAP1/MGCRACGAP antibody (ab2270) at 1 µg/ml + K562 cell lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 71 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
