Human Platelet Glycoprotein IIb + IIIa ELISA Kit (ab108851)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 1.25 ng/ml - 40 ng/ml
- Sample type: Cell Lysate, Plasma, Platelets
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Platelet Glycoprotein IIb + IIIa ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.4% Inter-assay Sample n Mean SD CV% Overall 8.2% -
Sample type
Plasma, Platelets, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.5 ng/ml -
Range
1.25 ng/ml - 40 ng/ml -
Recovery
101 %
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Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Platelet Glycoprotein IIb + IIIa Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Platelet Glycoprotein IIb+IIIa concentrations in Human platelets, platelet-rich plasma and cell culture lysate.
A Platelet Glycoprotein llb + llla specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Platelet Glycoprotein llb+llla specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Platelet Glycoprotein llb + llla captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Platelet Glycoprotein IIb + IIIa Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Platelet Glycoprotein IIb + IIIa Microplate (12 x 8 well strips) 1 unit Platelet Glycoprotein IIb + IIIa Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Alternative names
- GPIIb/IIIa
- Integrin alpha IIb beta 3
- Platelet membrane glycoprotein IIb/IIIa
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Database links
- Entrez Gene: 3690 Human
- Entrez Gene: 3674 Human
- SwissProt: P08514 Human
- SwissProt: P05106 Human
- Unigene: 411312 Human
- Unigene: 218040 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab108851.