Human PAPPA Antibody Pair - BSA and Azide free (ab244133)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human PAPPA Antibody Pair - BSA and Azide free
See all PAPP A kits -
Assay type
ELISA set -
Range
156.25 pg/ml - 14000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human PAPPA. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab235647), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human PAPPA Capture Antibody (unconjugated) 1 x 100µg Human PAPPA Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Metalloproteinase which specifically cleaves IGFBP-4 and IGFBP-5, resulting in release of bound IGF. Cleavage of IGFBP-4 is dramatically enhanced by the presence of IGF, whereas cleavage of IGFBP-5 is slightly inhibited by the presence of IGF. -
Tissue specificity
High levels in placenta and pregnancy serum. In placenta, expressed in X cells in septa and anchoring villi, and in syncytiotrophoblasts in the chorionic villi. Lower levels are found in a variety of other tissues including kidney, myometrium, endometrium, ovaries, breast, prostate, bone marrow, colon, fibroblasts and osteoblasts. -
Sequence similarities
Belongs to the peptidase M43B family.
Contains 5 Sushi (CCP/SCR) domains. -
Developmental stage
Present in serum and placenta during pregnancy; levels increase throughout pregnancy. -
Post-translational
modificationsThere appear to be no free sulfhydryl groups. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ASBABP2
- Aspecific BCL2 ARE binding protein 2
- Differentially placenta 1 expressed protein
see all -
Database links
- Entrez Gene: 5069 Human
- Omim: 176385 Human
- SwissProt: Q13219 Human
- Unigene: 643599 Human
Images
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Sandwich ELISA - Human PAPPA Antibody Pair - BSA and Azide free (ab244133)To learn more about the advantages of recombinant antibodies see here.
