Human Factor V ELISA Kit (ab137976)
Key features and details
- Sensitivity: 0.9 ng/ml
- Range: 0.938 ng/ml - 60 ng/ml
- Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Plasma, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Factor V ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.9% Inter-assay Sample n Mean SD CV% Overall 7.2% -
Sample type
Cell culture supernatant, Milk, Urine, Plasma, Cerebral Spinal Fluid -
Assay type
Sandwich (quantitative) -
Sensitivity
> 0.9 ng/ml -
Range
0.938 ng/ml - 60 ng/ml -
Recovery
97 %
Sample specific recovery Sample type Average % Range Milk 91% - 103% Urine 94% - 102% Plasma 94% - 104% -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Factor V Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor V in plasma, urine, milk, and cell culture supernatants.
A Factor V specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor V specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor V captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor V Antibody 1 x 120µl Chromogen Substrate 1 x 8ml Factor V Microplate (12 x 8 well strips) 1 unit Factor V Standard 1 vial Sealing Tapes 3 units Stop Solution 1 x 12ml -
Research areas
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Function
Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin. -
Tissue specificity
Plasma. -
Involvement in disease
Factor V deficiency
Thrombophilia due to activated protein C resistance
Budd-Chiari syndrome
Ischemic stroke
Pregnancy loss, recurrent, 1 -
Sequence similarities
Belongs to the multicopper oxidase family.
Contains 3 F5/8 type A domains.
Contains 2 F5/8 type C domains.
Contains 6 plastocyanin-like domains. -
Domain
Domain B contains 35 x 9 AA tandem repeats, and 2 x 17 AA repeats. -
Post-translational
modificationsThrombin activates factor V proteolytically to the active cofactor, factor Va (formation of a heavy chain at the N-terminus and a light chain at the C-terminus).
Sulfation is required for efficient thrombin cleavage and activation and for full procoagulant activity.
Activated protein C inactivates factor V and factor Va by proteolytic degradation.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Activated protein C cofactor
- APC cofactor
- Coagulation factor V
see all -
Database links
- Entrez Gene: 2153 Human
- Omim: 612309 Human
- SwissProt: P12259 Human
- Unigene: 30054 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab137976