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Human PAI1 ELISA Kit (ab157528)

Key features and details

  • Sensitivity: 0.11 U/ml
  • Range: 0.125 U/ml - 100 U/ml
  • Sample type: Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human PAI1 ELISA Kit
    See all PAI1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 20 2.8U/ml 0.257 9.18%
    2 20 6.82U/ml 0.323 4.74%
    3 20 29.6U/ml 2.57 8.68%
    Inter-assay
    Sample n Mean SD CV%
    1 10 4.2U/ml 0.399 9.52%
    2 10 8.07U/ml 0.763 9.46%
    3 10 26.8U/ml 2.1 7.85%

  • Sample type

    Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    = 0.11 U/ml

  • Range

    0.125 U/ml - 100 U/ml

  • Recovery

    96.45 %

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Abcam’s PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in Human plasma.


    PAI1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.


    Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate or be detected. Unbound PAI1 samples are aspirated and an anti-PAI1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.


    Get results in 90 minutes with Human PAI1 ELISA Kit (SERPINE1) (ab184863) from our SimpleStep ELISA® range.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-Human PAI1 Primary Antibody 1 vial
    Assay Diluent 1 x 10ml
    HRP secondary antibody 1 vial
    Human PAI-1 high activity standard (260U) 1 vial
    Human PAI-1 zero unit activity standard (0 U) 2 vials
    TMB Substrate Solution 1 x 10ml
    uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 unit

  • Research areas

  • Function

    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

  • Tissue specificity

    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

  • Involvement in disease

    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.

  • Sequence similarities

    Belongs to the serpin family.

  • Post-translational
    modifications

    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Clade E
    • Endothelial plasminogen activator inhibitor
    • Nexin
    • Nexin plasminogen activator inhibitor type 1
    • PAI
    • PAI 1
    • PAI-1
    • PAI1_HUMAN
    • PLANH1
    • Plasminogen activator inhibitor 1
    • Plasminogen activator inhibitor type 1
    • Serine (or cysteine) proteinase inhibitor
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serine proteinase inhibitor clade E member 1
    • serpin
    • Serpin E1
    • Serpin peptidase inhibitor clade E
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1
    • Serpine 1
    • SERPINE1
    see all

  • Database links

    Images

    • Typical Standard Cuve for ab157528 PAI-1 Human ELISA Linear Range
      Typical Standard Cuve for ab157528 PAI-1 Human ELISA Linear Range

      Typical Standard Cuve for ab157528 PAI-1 Human ELISA Linear Range

    • Typical Standard Cuve for ab157528 PAI-1 Human ELISA Full range
      Typical Standard Cuve for ab157528 PAI-1 Human ELISA Full range

      Typical Standard Cuve for ab157528 PAI-1 Human ELISA Full range

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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