Human PAI1 ELISA Kit (ab157528)
Key features and details
- Sensitivity: 0.11 U/ml
- Range: 0.125 U/ml - 100 U/ml
- Sample type: Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human PAI1 ELISA Kit
See all PAI1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 20 2.8U/ml 0.257 9.18% 2 20 6.82U/ml 0.323 4.74% 3 20 29.6U/ml 2.57 8.68% Inter-assay Sample n Mean SD CV% 1 10 4.2U/ml 0.399 9.52% 2 10 8.07U/ml 0.763 9.46% 3 10 26.8U/ml 2.1 7.85% -
Sample type
Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 0.11 U/ml -
Range
0.125 U/ml - 100 U/ml -
Recovery
96.45 %
-
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s PAI1 ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for is for the quantitative determination of active plasminogen activator inhibitor type 1 in Human plasma.
PAI1 is involved in the regulation of the blood fibrinolytic system. Increased plasma levels of PAI1 are implicated in the impairment of fibrinolytic function and may be associated with thrombotic diseases. Levels of PAI1 increase with age and are elevated in conditions such as normal pregnancy and sepsis.
Functionally active PAI-1 present in plasma reacts with urokinase coated and dried on a microtiter plate. Latent or complexed PAI1 will not bind to the plate or be detected. Unbound PAI1 samples are aspirated and an anti-PAI1 primary antibody is added. Excess primary antibody is then aspirated. The bound antibody, which is proportional to the original active PAI1 present in the samples, is then reacted with the horseradish peroxidase conjugated secondary antibody. Following an additional washing step, TMB substrate solution is then used for color development at 450nm. The amount of color development is directly proportional to the concentration of active PAI1 in the sample.
Get results in 90 minutes with Human PAI1 ELISA Kit (SERPINE1) (ab184863) from our SimpleStep ELISA® range.
-
Platform
Microplate
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer 1 x 50ml Anti-Human PAI1 Primary Antibody 1 vial Assay Diluent 1 x 10ml HRP secondary antibody 1 vial Human PAI-1 high activity standard (260U) 1 vial Human PAI-1 zero unit activity standard (0 U) 2 vials TMB Substrate Solution 1 x 10ml uPA Antibody pre-coated 96 well microplate (12 x 8 well strips) 1 unit -
Research areas
-
Function
This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. -
Tissue specificity
Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells. -
Involvement in disease
Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
Sequence similarities
Belongs to the serpin family. -
Post-translational
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
Cellular localization
Secreted. - Information by UniProt
-
Alternative names
- Clade E
- Endothelial plasminogen activator inhibitor
- Nexin
see all -
Database links
- Entrez Gene: 5054 Human
- Omim: 173360 Human
- SwissProt: P05121 Human
- Unigene: 414795 Human
- Unigene: 713079 Human
Images
-
Typical Standard Cuve for ab157528 PAI-1 Human ELISA Linear Range
Typical Standard Cuve for ab157528 PAI-1 Human ELISA Linear Range
-
Typical Standard Cuve for ab157528 PAI-1 Human ELISA Full rangeTypical Standard Cuve for ab157528 PAI-1 Human ELISA Full range
