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Human NUP210 (GP210) knockout HEK-293T cell lysate (ab258549)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human NUP210 (GP210) knockout HEK-293T cell lysate (ab258549)
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  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
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Overview

  • Product name

    Human NUP210 (GP210) knockout HEK-293T cell lysate
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon5 and 1 bp insertion in exon5 and 28 bp deletion in exon5.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261272 - Human NUP210 knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Target

  • Function

    Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
  • Tissue specificity

    Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
  • Sequence similarities

    Belongs to the NUP210 family.
  • Post-translational
    modifications

    N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides.
    Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
  • Cellular localization

    Nucleus > nuclear pore complex. Nucleus membrane. Endoplasmic reticulum membrane.
  • Target information above from: UniProt accession Q8TEM1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • FLJ22389
    • GP 210
    • KIAA0906
    • Nuclear envelope pore membrane protein POM 210
    • Nuclear pore membrane glycoprotein 210
    • Nuclear pore protein gp210
    • Nucleoporin 210
    • Nucleoporin 210kDa
    • Nucleoporin Nup210
    • Nucleoporin210
    • NUP 210
    • Nup210
    • PO210_HUMAN
    • POM 210
    • POM210
    • Pore membrane protein of 210 kDa
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab261272 - Human NUP210 knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Nuclear Pore
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Images

  • Sanger Sequencing - Human NUP210 knockout HEK293T cell lysate (ab258549)
    Sanger Sequencing - Human NUP210 knockout HEK293T cell lysate (ab258549)

    Allele-1: 28 bp deletion in exon5

     

  • Sanger Sequencing - Human NUP210 knockout HEK293T cell lysate (ab258549)
    Sanger Sequencing - Human NUP210 knockout HEK293T cell lysate (ab258549)

    Allele-2: 1 bp deletion in exon5

     

  • Sanger Sequencing - Human NUP210 knockout HEK293T cell lysate (ab258549)
    Sanger Sequencing - Human NUP210 knockout HEK293T cell lysate (ab258549)

    Allele-3: 1 bp insertion in exon5

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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