Human NT-ProANP ELISA Kit (ab245705)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 26.2 pg/ml
- Range: 78.1 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human NT-ProANP ELISA Kit
See all Natriuretic peptides A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% plasma 8 4.1% Inter-assay Sample n Mean SD CV% plasma 3 1.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
26.2 pg/ml -
Range
78.1 pg/ml - 5000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 85 83% - 87% Cell culture media 102 96% - 106% Hep Plasma 91 83% - 96% EDTA Plasma 95 93% - 98% Cit plasma 83 82% - 84% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human NT-ProANP ELISA Kit (ab245705) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of NT-ProANP protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human NT-ProANP with 26.2 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human NT-ProANP Capture Antibody 1 x 600µl 10X Human NT-ProANP Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human NT-ProANP Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. -
Involvement in disease
Atrial standstill 2
Atrial fibrillation, familial, 6 -
Sequence similarities
Belongs to the natriuretic peptide family. -
Post-translational
modificationsCleaved by CORIN upon secretion to produce the functional hormone.
Atrial natriuretic factor: Cleaved by MME. The cleavage initiates degradation of the factor and thereby regulate its activity. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ANF
- ANF_HUMAN
- ANP
see all -
Database links
- Entrez Gene: 4878 Human
- Omim: 108780 Human
- SwissProt: P01160 Human
- Unigene: 75640 Human
Images
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Other - Human NT-ProANP ELISA Kit (ab245705)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human NT-ProANP Standard Curve ComparisonStandard Curve comparison between human NT-ProANP SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity. -
Example of human NT-ProANP standard curve in Sample Diluent NS.The NT-ProANP standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed. -
Interpolated concentrations of native NT-ProANP in human plasma samples.The concentrations of NT-ProANP were measured in duplicates, interpolated from the NT-ProANP standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 100%, plasma (heparin) 100%, and plasma (EDTA) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-ProANP concentration was determined to be 713.8 pg/mL in neat plasma (citrate), 2,675 pg/mL in neat plasma (heparin), 5,029 pg/mL in neat plasma (EDTA). -
Serum from ten individual apparently healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean NT-ProANP concentration was determined to be 10.76 ng/mL (based on detectable samples) with a range of undetectable - 15.68 ng/mL. Serum from ten individual apparently healthy human male donors was also measured in duplicate; all values were below the detectable range of the assay (not shown).
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Sandwich ELISA - Human NT-ProANP ELISA Kit (ab245705)To learn more about the advantages of recombinant antibodies see here.
