ADP Assay Kit (Colorimetric/Fluorometric) (ab83359)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Cell culture media, Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 1 µM
Overview
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Product name
ADP Assay Kit (Colorimetric/Fluorometric)
See all ADP kits -
Detection method
Colorimetric/Fluorometric -
Sample type
Urine, Serum, Plasma, Cell culture extracts, Other biological fluids, Tissue Extracts, Cell Lysate, Cell culture media -
Assay type
Quantitative -
Sensitivity
> 1 µM -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
ADP Assay Kit (Colorimetric/Fluorometric) (ab83359) provides a convenient colorimetric and fluorometric method to measure ADP level.
In the ADP assay, ADP is converted to ATP and pyruvate. The generated pyruvate can be quantified by colorimetric (ODmax = 570 nm) or fluorometric method (Ex/Em 535/587 nm).
Conventionally, ADP levels are measured by luciferase/luciferin mediated assays after ADP is converted to ATP. However, the luciferase system is unstable and luminescence equipment is not generally available in most laboratories. In comparison, this assay is simple, sensitive, stable and high-throughput adaptable and can be used with conventional microplate readers.
The ADP assay can detect as low as 1 μM ADP in biological samples.
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Notes
ADP is a product of ATP de-phosphorylation and it can be rephosphorylated to ATP. De-phosphorylation and re-phosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests ADP Assay Buffer WM 1 x 25ml ADP Converter (lyophilized) Purple 1 vial ADP Developer Mix (lyophilized) Green 1 vial ADP Probe (in DMSO) Red 1 x 200µl ADP Standard (1 µmole) (lyophilized) Yellow 1 vial -
Research areas
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Relevance
ADP (Adenosine diphosphate) is a product of ATP dephosphorylation and it can be rephosphorylated to ATP. Dephosphorylation and rephosphorylation occur via various phosphatases, phosphorylases and kinases. ADP is stored in platelets and can be released to interact with a variety of purinergic receptors. ADP levels regulate several enzymes involved in intermediary metabolism. ADP conversion to ATP primarily occurs within the mitochondrion and chloroplast although several such processes occur in the cytoplasm. -
Alternative names
- Adenosine diphosphate
Images
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Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)
ADP levels measured fluorometrically in cell culture supernatants (background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)ADP levels measured fluorometrically in cell lysates (background signal subtracted, mean of duplicates; +/- SD).
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Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)APD levels colorimetrically measured in biological fluids (rat plasma and serum, human saliva); background signal subtracted, mean of duplicates; +/- SD.
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Functional Studies - ADP Colorimetric/Fluorometric Assay Kit (ab83359)Colorimetric and Fluorometric examples of ADP Standard curve.
