Human MASP2 Antibody Pair - BSA and Azide free (ab253506)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human MASP2 Antibody Pair - BSA and Azide free
See all MASP2 kits -
Assay type
ELISA set -
Range
125 pg/ml - 8000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human MASP2 Antibody Pair is a matched pair of unconjugated recombinant rabbit monoclonal capture and detection antibodies used to quantify Human MASP2 in sandwich ELISAs and many other pair-based applications.
The pair can be used in variety of assays and platforms including but not limited to:
- Sandwich ELISA
- FRET/TR-FRET/HTR
- Meso Scale Discovery® (MSD®)
- Luminex® and bead-based assays
- AlphaLISA®/AlphaScreen®
- DELFIA® immunoassays
- Simoa® and Single Molecule Counting (SMC™) immunoassays
- MultiplexOur antibody pairs are supplied in a carrier-free format that is conjugation-ready:
- Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
- Concentration of ~1 mg/ml as measured by the protein A280 method.Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody: recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody: recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration: ~1 mg/ml
Storage buffer: 100% PBS
Form: Liquid
Isotype: IgG
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free productionFor more information see here.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
Luminex is a trademark of Luminex Corporation, registered in the US and other countries.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany. -
Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components Identifier 10 x 96 tests 10X Mouse IGFBP5 Capture Antibody — 10X Mouse IGFBP5 Detector Antibody — -
Research areas
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Relevance
MASP2, MBL associated serine protease 2, is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin (MBL). Binding of MBL to carbohydrate groups triggers MASP2 activation by auto-catalytic cleavage which leads to cleavage of C2 and C4, leading to their activation and to the formation of C3 convertase. MASP2 also binds to L-Ficolin and H-Ficolin, and when the complex binds pathogens the MASP is activated. Low MBL levels in serum are associated with increased susceptibility to infection, and an Asp105Gly mutation in MASP2 is associated with low MASP2 levels in serum. Reduced functional levels of MBL are also postulated to be associated with generation of autoantibodies in systemic lupus erythematosus, acting through a decreased clearance of apoptotic material. A truncated form of MASP2 that constitutes the amino 185 residues, Map19 is also a component of the MBL-MASP-1 complex. -
Cellular localization
Secreted -
Alternative names
- Mannan binding lectin serine peptidase 2
- Mannan binding lectin serine protease 2
- Mannose binding protein associated serine protease 2
see all -
Database links
- Entrez Gene: 10747 Human
- Omim: 605102 Human
- SwissProt: O00187 Human
- Unigene: 655645 Human
