Human MASP2 ELISA Kit (ab278121)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 21 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MASP2 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 24 4.7% Inter-assay Sample n Mean SD CV% Serum 3 5.4% -
Sample type
Serum, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
21 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 99 87% - 111% Hep Plasma 103 97% - 114% EDTA Plasma 99 86% - 106% Cit plasma 91 80% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MASP2 ELISA kit (ab278121) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human MASP2 protein in human serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MASP2 with 21 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MASP2, Mannan-binding lectin serine protease 2, plays a key role in the complement activation cascade. This protein is part of a larger mannose-binding complex which senses foreign material, activates C3 convertase, and perpetuates the immune response. The immunogen from which the antibodies in this kit were raised represents the N-terminal half of the protein. This protein does not share high sequence identity in mouse or rat samples (80 and 81%, respectively) and, therefore, this kit is not expected to cross-react.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MASP2 Capture Antibody 1 x 600µl 10X Human MASP2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human MASP2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
MASP2, MBL associated serine protease 2, is a serum protease that plays an important role in the activation of the complement system via mannose-binding lectin (MBL). Binding of MBL to carbohydrate groups triggers MASP2 activation by auto-catalytic cleavage which leads to cleavage of C2 and C4, leading to their activation and to the formation of C3 convertase. MASP2 also binds to L-Ficolin and H-Ficolin, and when the complex binds pathogens the MASP is activated. Low MBL levels in serum are associated with increased susceptibility to infection, and an Asp105Gly mutation in MASP2 is associated with low MASP2 levels in serum. Reduced functional levels of MBL are also postulated to be associated with generation of autoantibodies in systemic lupus erythematosus, acting through a decreased clearance of apoptotic material. A truncated form of MASP2 that constitutes the amino 185 residues, Map19 is also a component of the MBL-MASP-1 complex. -
Cellular localization
Secreted -
Alternative names
- Mannan binding lectin serine peptidase 2
- Mannan binding lectin serine protease 2
- Mannose binding protein associated serine protease 2
see all -
Database links
- Entrez Gene: 10747 Human
- Omim: 605102 Human
- SwissProt: O00187 Human
- Unigene: 655645 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Recombinant Antibody benefitsTo learn more about the advantages of recombinant antibodies see here.
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Example of human MASP2 standard curve in Sample Diluent NS.The MASP2 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MASP2 in human serum and plasma samples.The concentrations of MASP2 were measured in duplicates, interpolated from the MASP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (heparin) 1% and plasma (EDTA) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASP2 concentration was determined to be 484.87 ng/mL in serum, 561.89 ng/mL in plasma (citrate), 500.49 ng/mL in plasma (heparin), and 404.43 ng/mL in plasma (EDTA).
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MASP2 concentration was determined to be 343.24 ng/mL with a range of 56.31 – 528.11 ng/mL.
