Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)
![Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)](https://www.abcam.com/ps/products/19/ab19079/Images/ab19079-28598-anti-fibrinogen-alpha-chain-antibody-uc45-flow-cytometry.jpg "Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)")
Key features and details
- Mouse monoclonal [UC45] to Fibrinogen alpha chain
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgM
Overview
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Product name
Anti-Fibrinogen alpha chain antibody [UC45]
See all Fibrinogen alpha chain primary antibodies -
Description
Mouse monoclonal [UC45] to Fibrinogen alpha chain -
Host species
Mouse -
Specificity
This antibody reacts very strongly with the alpha chain of human fibrinogen, for further information please see Hogg 1983 paper quoted. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Human acute monoblastic leukemia cells.
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General notes
A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from tissue culture supernatant. -
Primary antibody notes
A monocyte and neuronal antigen present predominantly on a protein of 45kDa MW. UC45 is specific for monocytic leukemias. The relevant antigens are not expressed in all cases with monocytic differentiation. -
Clonality
Monoclonal -
Clone number
UC45 -
Myeloma
P3-NS1/1-Ag4-1 -
Isotype
IgM -
Research areas
Images
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Flow Cytometry - Anti-Fibrinogen alpha chain antibody [UC45] (ab19079)Overlay histogram showing THP1 cells stained with ab19079 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab19079, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) (ab97007) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
