HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)
![HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)](https://www.abcam.com/ps/products/40/ab40742/Images/ab40742-53802-anti-alpha-tubulin-antibody-dm1a-loading-control-hrp-western-blot.jpg "HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)")
Key features and details
- HRP Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human, Xenopus laevis
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control
See all alpha Tubulin primary antibodies -
Description
HRP Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Xenopus laevis
Predicted to work with: Guinea pig
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Immunogen
Full length native protein (purified) corresponding to Chicken alpha Tubulin.
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Positive control
- This antibody gave a positive signal in the following: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Brain (Rat) Tissue Lysate - normal tissue Xenopus (Blastula) Whole Cell Lysate. IHC-P: FFPE human colon (normal) tissue sections.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
IgG fraction -
Clonality
Monoclonal -
Clone number
DM1A -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)All lanes : HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742) at 1/5000 dilution
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :NIH/3T3 whole cell lysate (ab7179)
Lane 3 : Brain (Rat) Tissue Lysate
Lane 4 : Xenopus Laevis Protein Extracts (Stg 8)
Lysates/proteins at 10 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutes -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)](https://www.abcam.com/ps/products/40/ab40742/Images/ab40742-254078-anti-alpha-tubulin-antibody-dm1a-loading-control-hrp-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab40742)IHC image of alpha Tubulin staining in a section of formalin-fixed paraffin-embedded normal human colon tissue*, performed on a Leica BONDTM. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab40742, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
