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Epigenetics and Nuclear Signaling Transcription Domain Families Developmental Families HOX

Human KDM6B (JMJD3) knockout HEK-293T cell lysate (ab257091)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human KDM6B (JMJD3) knockout HEK-293T cell lysate (ab257091)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human KDM6B (JMJD3) knockout HEK-293T cell lysate
    See all KDM6B / JMJD3 kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    HEK293T
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 5.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260174 - Human KDM6B knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Target

  • Function

    Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.
  • Sequence similarities

    Belongs to the UTX family.
    Contains 1 JmjC domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O15054 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • Histone demethylase JMJD3
    • JmjC domain containing protein 3
    • JmjC domain-containing protein 3
    • Jumonji D3
    • Jumonji domain containing 3
    • Jumonji domain containing 3 histone lysine demethylase
    • Jumonji domain containing protein 3
    • Jumonji domain-containing protein 3
    • Kdm6b
    • KDM6B_HUMAN
    • KIAA0346
    • Lysine demethylase 6B
    • Lysine K specific demethylase 6B
    • Lysine specific demethylase 6B
    • Lysine-specific demethylase 6B
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab260174 - Human KDM6B knockout HEK293T cell lysate 1 x 100µg
    ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • HOX
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Jumonji containing proteins
  • Cell type

    epithelial
  • STR Analysis

    Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12

Images

  • Sanger Sequencing - Human KDM6B knockout HEK293T cell lysate (ab257091)
    Sanger Sequencing - Human KDM6B knockout HEK293T cell lysate (ab257091)
    Homozygous: 14 bp deletion in exon 5

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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