Human IRAK-1 Matched Antibody Pair Kit (ab220136)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human IRAK-1 Matched Antibody Pair Kit
See all IRAK-1 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
2.5 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human IRAK-1 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human IRAK-1.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219630. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human IRAK-1 Capture Antibody 2 x 50µg 1 x 50µg Human IRAK-1 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human IRAK-1 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. -
Tissue specificity
Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2. -
Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated or is transphosphorylated by IRAK4 following recruitment to the IL-1RI. In the case of isoform 1, this is linked to ubiquitination and degradation.
Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. - Information by UniProt
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Alternative names
- AA48924
- Il1rak
- Interleukin 1 receptor associated kinase 1
see all -
Database links
- Entrez Gene: 3654 Human
- Omim: 300283 Human
- SwissProt: P51617 Human
- Unigene: 522819 Human
Images
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Human IRAK-1 standard curve.
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Sandwich ELISA - Human IRAK-1 Matched Antibody Pair Kit (ab220136)To learn more about the advantages of recombinant antibodies see here.
