Anti-FKBP135 antibody (ab14432)
Key features and details
- Rabbit polyclonal to FKBP135
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FKBP135 antibody
See all FKBP135 primary antibodies -
Description
Rabbit polyclonal to FKBP135 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human FKBP135 aa 50-100.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was purified using an epitope specific to FKBP-135 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FKBP135 antibody (ab14432)All lanes : Anti-FKBP135 antibody (ab14432) at 0.2 µg/ml
Lane 1 : Nuclear lysates prepared from Hela cells at 5 µg
Lane 2 : Nuclear lysates prepared from Hela cells at 20 µg
Lanes 3-5 : Nuclear lysates prepared from Hela cells at 1 mg/ml
Predicted band size: 135 kDaAntibodies: ab14432 used at 0.2 mcg/ml for WB in A and B and 2 mcg/mg extract for IP (B), ab14433 and ab14434 used at 2 mcg/mg extract for IP. Detection: Chemiluminescence with an exposure time of 25 minutes (A) and 10 minutes (B).
In figure B the Immunoprecipitation was done using ab14432 (Lane 3), ab14433 (Lane 4) and ab14434 (Lane 5) at 2µg/mg of extract.
