Human Interferon gamma Receptor 1 ELISA Kit (CD119) (ab173193)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 140 pg/ml
- Range: 0.14 ng/ml - 100 ng/ml
- Sample type: Adherent cells, Cell culture extracts, Cell Lysate, Suspension cells, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Interferon gamma Receptor 1 ELISA Kit (CD119)
See all IFNGR1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% MCF7 Lysate 9 4.9% Inter-assay Sample n Mean SD CV% MCF7 Lysate 3 6.2% -
Sample type
Cell culture extracts, Adherent cells, Suspension cells, Cell Lysate, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
140 pg/ml -
Range
0.14 ng/ml - 100 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 82 72% - 87% Plasma 78 73% - 85% Cell culture media 84 80% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Interferon-gamma Receptor 1 (CD119) ELISA kit (ab173194) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Interferon-gamma Receptor 1 protein in cell lysates and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate human Interferon-gamma Receptor 1 with 140 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits. -
Notes
Interferon gamma receptor 1 (IFNGR1) is a heterodimeric receptor that binds one interferon gamma dimer as a ligand. The receptor is a single-pass type I membrane protein present on the plasma membrane. A genetic variation in the IFNGR1 gene is associated with susceptibility to Helicobacter pylori infection. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD); also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IFNGR1 Capture Antibody 1 x 600µl 10X Human IFNGR1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5B 1 x 6ml Human IFNGR1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Receptor for interferon gamma. Two receptors bind one interferon gamma dimer. -
Involvement in disease
Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD) [MIM:209950]; also known as familial disseminated atypical mycobacterial infection. This rare condition confers predisposition to illness caused by moderately virulent mycobacterial species, such as Bacillus Calmette-Guerin (BCG) vaccine and environmental non-tuberculous mycobacteria, and by the more virulent Mycobacterium tuberculosis. Other microorganisms rarely cause severe clinical disease in individuals with susceptibility to mycobacterial infections, with the exception of Salmonella which infects less than 50% of these individuals. The pathogenic mechanism underlying MSMD is the impairment of interferon-gamma mediated immunity whose severity determines the clinical outcome. Some patients die of overwhelming mycobacterial disease with lepromatous-like lesions in early childhood, whereas others develop, later in life, disseminated but curable infections with tuberculoid granulomas. MSMD is a genetically heterogeneous disease with autosomal recessive, autosomal dominant or X-linked inheritance. -
Sequence similarities
Belongs to the type II cytokine receptor family.
Contains 2 fibronectin type-III domains.
Contains 2 Ig-like C2-type (immunoglobulin-like) domains. -
Post-translational
modificationsPhosphorylated at Ser/Thr residues. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Antiviral Protein Type II
- Antiviral protein, type 2
- AVP type II
see all -
Database links
- Entrez Gene: 3459 Human
- Omim: 107470 Human
- SwissProt: P15260 Human
- Unigene: 520414 Human
Images
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Other - Human Interferon gamma Receptor 1 ELISA Kit (CD119) (ab173193)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Typical Standard CurveExample IFNGR1 standard curve. Background-subtracted data values (mean +/- SD) are graphed. A new standard curve must be generated for each assay performed.
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ELISA typical dataTitration of MCF7 cell lysate within the working range of the assay. Background subtracted data from triplicate measurements are plotted.
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Example dataQuantification of IFNGR1 expression in different mammalian cell lines. Interpolated values of IFNGR1 are plotted for the indicated cell lines based on a lysate concentration of 100 µg/mL.