Human IL-17A ELISA Kit (ab216167)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.1 pg/ml
- Range: 1.56 pg/ml - 100 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-17A ELISA Kit
See all IL-17A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 3 10.1% Inter-assay Sample n Mean SD CV% Overall 5 6.8% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.1 pg/ml -
Range
1.56 pg/ml - 100 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 97 97% - 98% Cell culture media 92 82% - 108% Hep Plasma 96 86% - 115% EDTA Plasma 106 104% - 110% Cit plasma 101 94% - 108% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human IL-17A ELISA Kit (ab216167) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of IL-17A protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human IL-17A with 1.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
IL-17A is a proinflammatory cytokine that is secreted by activated T cells. It is a disulfide-linked homodimer with both glycosylated and nonglycosylated forms. IL-17A induces stromal cells to produce proinflammatory and hematopoietic cytokines, and also enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human IL-17A Capture Antibody 1 x 600µl 10X Human IL-17A Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BI 1 x 6ml Human IL-17A Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CTLA 8
- CTLA-8
- CTLA8
see all -
Database links
- Entrez Gene: 3605 Human
- Omim: 603149 Human
- SwissProt: Q16552 Human
- Unigene: 41724 Human
Images
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Other - Human IL-17A ELISA Kit (ab216167)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human IL-17A standard curve comparison data.Standard curve comparison between human IL-17A SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows a 13-fold increase in sensitivity.
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Example of human IL-17A standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native IL-17A in PHA-M stimulated and unstimulated human PBMC cell culture supernatant samples.The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: stimulated PBMC supernatant 10%, and unstimulated PBMC supernatant 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean IL-17A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC supernatant and 43.6 pg/mL in neat unstimulated PBMC supernatant.
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Interpolated concentrations of spike IL-17A in human serum and plasma samples.The concentrations of IL-17A were measured in duplicates, interpolated from the IL-17A standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (heparin) 50%, plasma (EDTA) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Comparison of IL-17A in unstimulated and PHA-M stimulated human PBMC cell supernatants.Human PBMC cells were cultured in the absence or presence of 1.5% PHA-M for 46 hours. The concentrations of IL-17A were measured in supernatant samples in 5 different dilutions in duplicates and interpolated from the IL-17 A standard curve. The interpolated values are plotted (mean +/- SD, n=5). The mean IL-17 A concentration was determined to be 542 pg/mL in neat PHA-M stimulated PBMC cell supernatant, 43.6 pg/mL in neat unstimulated supernatants and undetectable in neat media (not shown).
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Sandwich ELISA - Human IL-17A ELISA Kit (ab216167)To learn more about the advantages of recombinant antibodies see here.
