Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)
![Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)](https://www.abcam.com/ps/products/256/ab256167/Images/ab256167-2-anti-il-6r-antibody-epr22628-236-immunoprecipitation-u266b1-human.jpg "Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22628-236] to IL-6R - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free
See all IL-6R primary antibodies -
Description
Rabbit monoclonal [EPR22628-236] to IL-6R - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: U266B1 whole cell lysate. Flow cyt: U937 cells.
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General notes
Ab256167 is the carrier-free version of ab222101. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab256167 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22628-236 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)
IL-6R was immunoprecipitated from 0.35 mg of U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate with ab222101 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222101 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.
Lane 1: U266B1 whole cell lysate 10 μg (Input).
Lane 2: ab222101 IP in U266B1 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222101 in U266B1 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 15 seconds.The molecular weight observed is consistent with what has been described in the literature (PMID: 28276471; PMID: 28060820).
Bands between 40-70KD are likely to be cleaved forms (PMID: 28060820).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222101).
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![Flow Cytometry - Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)](https://www.abcam.com/ps/products/256/ab256167/Images/ab256167-1-anti-il-6r-antibody-epr22628-236-flowcytometry-k562-u937-human.jpg)
Flow Cytometry - Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) (Left) and U937 (human histiocytic lymphoma cell line) (Right) cells labeling IL-6R with ab222101 at 1/50 (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.
Negative control: K562 (PMID: 8300137)
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222101).
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![Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)](https://www.abcam.com/ps/products/256/ab256167/Images/ab256167-3-benefits-of-recombinant-antibodies.png)
Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)
