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Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22628-236] to IL-6R - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free
    See all IL-6R primary antibodies

  • Description

    Rabbit monoclonal [EPR22628-236] to IL-6R - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: U266B1 whole cell lysate. Flow cyt: U937 cells.

  • General notes

    Ab256167 is the carrier-free version of ab222101. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab256167 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)
    Immunoprecipitation - Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)

    IL-6R was immunoprecipitated from 0.35 mg of U266B1 (human multiple myeloma B lymphocyte cell line) whole cell lysate with ab222101 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab222101 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used as secondary antibody at 1/5000 dilution.

    Lane 1: U266B1 whole cell lysate 10 μg (Input).
    Lane 2: ab222101 IP in U266B1 whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab222101 in U266B1 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 15 seconds.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 28276471; PMID: 28060820).

    Bands between 40-70KD are likely to be cleaved forms (PMID: 28060820).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222101).

  • Flow Cytometry - Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)
    Flow Cytometry - Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)

    Flow cytometric analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) (Left) and U937 (human histiocytic lymphoma cell line) (Right) cells labeling IL-6R  with ab222101 at 1/50 (red) compared with Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG Fc (Alexa Fluor® 488) preadsorbed (ab150097), at 1/5000 dilution was used as the secondary antibody.

    Negative control: K562 (PMID: 8300137)

    Gated on viable cells.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab222101).

  • Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)
    Anti-IL-6R antibody [EPR22628-236] - BSA and Azide free (ab256167)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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