Human IL-12 p70 ELISA Kit, Fluorescent (ab229515)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 pg/ml
- Range: 3.9 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Fluorescent
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-12 p70 ELISA Kit, Fluorescent
See all IL-12 p70 kits -
Detection method
Fluorescent -
Precision
Intra-assay Sample n Mean SD CV% Spiked serum 5 7% Inter-assay Sample n Mean SD CV% Spiked serum 3 5.2% -
Sample type
Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1 pg/ml -
Range
3.9 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 96 93% - 101% Cell culture media 101 94% - 110% Hep Plasma 85 80% - 89% EDTA Plasma 99 97% - 101% Cit plasma 93 92% - 94% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
IL-12 p70 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 protein in human serum, plasma and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
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Notes
IL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits IL-12A (p35) and IL-12B (p40). This kit recognizes specifically the IL-12 total heterodimer protein. IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFNγ.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X Stoplight Red Substrate 1 x 120µl 10X Human IL-12 p70 Capture Antibody 1 x 600µl 10X Human IL-12 p70 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml Human IL-12 p70 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated Black 96-Well Microplate 1 unit Stoplight Red Substrate Buffer 1 x 12ml -
Research areas
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Relevance
IL-12 p70 is a disulfide bonded heterodimer of the IL-12 40 kDa and 35 kDa subunits. -
Cellular localization
Secreted -
Alternative names
- IL12A
- IL12B
- CLMF p35
see all -
Database links
- Entrez Gene: 3593 Human
- Entrez Gene: 3592 Human
- Omim: 161561 Human
- Omim: 161560 Human
- SwissProt: P29460 Human
- SwissProt: P29459 Human
- Unigene: 674 Human
- Unigene: 673 Human
Images
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Other - Human IL-12 p70 ELISA Kit, Fluorescent (ab229515)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human IL-12 p70 standard curve in Sample Diluent NS.The IL-12 p70 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spike IL-12 p70 in human serum, plasma and cell culture supernatant samples.The concentrations of IL-12 p70 were measured in duplicates, interpolated from the IL-12 p70 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, plasma (citrate) 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of IL-12 p70 was measured.
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Sandwich ELISA - Human IL-12 p70 ELISA Kit, Fluorescent (ab229515)To learn more about the advantages of recombinant antibodies see here.
