Human IL-12 p70 ELISA Kit (ab46143)
Key features and details
- Sensitivity: 2.2 pg/ml
- Range: 6.25 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-12 p70 ELISA Kit
See all IL-12 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 4.3% Inter-assay Sample n Mean SD CV% Overall 6 8.9% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
= 2.2 pg/ml -
Range
6.25 pg/ml - 200 pg/ml -
Recovery
95 %
Sample specific recovery Sample type Average % Range Serum 91 86% - 94% -
Assay time
3h 40m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-12 p70 (Interleukin- 12 p70) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 in supernatants, buffered solutions or serum and plasma samples.
A monoclonal antibody specific IL-12 p70 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-12 p70 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-12 p70 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-12 p70 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-12 p70.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml Biotinylated anti-IL-12 p70 Red 2 x 400µl 1 x 400µl Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml Control Silver 4 vials 2 vials HRP Diluent Red 1 x 23ml 1 x 23ml IL-12 p70 Microplate (12 x 8 well strips) 2 units 1 unit IL-12p70 Standard (Lyophilized) Yellow 4 vials 2 vials Standard Diluent (Serum) 1 x 7ml 2 x 7ml Stop Reagent Black 2 x 11ml 1 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl -
Research areas
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Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CLMF
- CLMF p35
- CLMF p40
see all -
Database links
- Entrez Gene: 3592 Human
- Entrez Gene: 3593 Human
- Omim: 161561 Human
- Omim: 161560 Human
- SwissProt: P29459 Human
- SwissProt: P29460 Human
- Unigene: 673 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab46143
