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Human IL-1 RL1 ELISA Kit (ST2) (ab213796)

Key features and details

  • Sensitivity: 10 pg/ml
  • Range: 156 pg/ml - 10000 pg/ml
  • Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human IL-1 RL1 ELISA Kit (ST2)
    See all ST2 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 0.53ng/ml 0.028 5.2%
    2 16 2.05ng/ml 0.094 4.6%
    3 16 4.31ng/ml 0.142 3.3%
    Inter-assay
    Sample n Mean SD CV%
    1 24 0.69ng/ml 0.06 8.7%
    2 24 2.54ng/ml 0.157 6.2%
    3 24 4.68ng/ml 0.229 4.9%

  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    10 pg/ml

  • Range

    156 pg/ml - 10000 pg/ml

  • Assay time

    3h 30m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    The Human IL-1 RL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ST2) (ab213796) is designed for the quantitative detection of Human IL1RL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA). 


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-1 RL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: K19-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL1RL1amount of sample captured in plate.

  • Notes

    Interleukin 1 receptor-like 1, also known as IL-1 RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by pro-inflammatory stimuli and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL-1 RL1), interleukin 1 receptor, type II (IL-1 R2) and interleukin 1 receptor like 2 (IL-1 RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human IL1RL1 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl
    Biotinylated anti-Human IL1RL1 antibody 1 x 130µl
    Lyophilized recombinant Human IL1RL1 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml

  • Research areas

  • Function

    Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.

  • Tissue specificity

    Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle.

  • Sequence similarities

    Belongs to the interleukin-1 receptor family.
    Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 TIR domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Alternative names

    • DER-4
    • DER4
    • FIT 1
    • Growth stimulation expressed
    • homolog of mouse growth stimulation-expressed
    • Il1rl1
    • IL33R
    • ILRL1_HUMAN
    • Interleukin 1 receptor like 1
    • interleukin 1 receptor related protein
    • Interleukin-1 receptor-like 1
    • Protein ST2
    • ST2
    • ST2L
    • ST2V
    • T1
    • T1 protein
    see all

  • Database links

    Images

    • Human IL1RL1 ELISA Kit (ST2) (ab213796) Standard Curve
      Human IL1RL1 ELISA Kit (ST2) (ab213796) Standard Curve

      Human IL1RL1 ELISA Kit (ST2) (ab213796) Standard Curve.

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