Human IL-1 RL1 ELISA Kit (ST2) (ab213796)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 156 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, EDTA Plasma, Hep Plasma, Serum, Tissue Homogenate
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-1 RL1 ELISA Kit (ST2)
See all ST2 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 0.53ng/ml 0.028 5.2% 2 16 2.05ng/ml 0.094 4.6% 3 16 4.31ng/ml 0.142 3.3% Inter-assay Sample n Mean SD CV% 1 24 0.69ng/ml 0.06 8.7% 2 24 2.54ng/ml 0.157 6.2% 3 24 4.68ng/ml 0.229 4.9% -
Sample type
Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate -
Assay type
Sandwich (quantitative) -
Sensitivity
10 pg/ml -
Range
156 pg/ml - 10000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human IL-1 RL1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ST2) (ab213796) is designed for the quantitative detection of Human IL1RL1 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for IL-1 RL1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: K19-S328) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1 RL1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL1RL1amount of sample captured in plate.
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Notes
Interleukin 1 receptor-like 1, also known as IL-1 RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by pro-inflammatory stimuli and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL-1 RL1), interleukin 1 receptor, type II (IL-1 R2) and interleukin 1 receptor like 2 (IL-1 RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human IL1RL1 coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human IL1RL1 antibody 1 x 130µl Lyophilized recombinant Human IL1RL1 standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Function
Receptor for interleukin-33 (IL-33), its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function. -
Tissue specificity
Highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. Isoform A is prevalently expressed in the lung, testis, placenta, stomach and colon. Isoform B is more abundant in the brain, kidney and the liver. Isoform C is not detected in brain, heart, liver, kidney and skeletal muscle. -
Sequence similarities
Belongs to the interleukin-1 receptor family.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 TIR domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Alternative names
- DER-4
- DER4
- FIT 1
see all -
Database links
- Entrez Gene: 9173 Human
- Omim: 601203 Human
- SwissProt: Q01638 Human
- Unigene: 66 Human
Images
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Human IL1RL1 ELISA Kit (ST2) (ab213796) Standard Curve
Human IL1RL1 ELISA Kit (ST2) (ab213796) Standard Curve.
