Human IKBKG (IKK gamma/NEMO) knockout HEK-293T cell lysate (ab257153)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260190 - Human IKBKG knockout HEK293T cell lysate 1 x 100µg ab255553 - Human wild-type HEK293T cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human IKBKG (IKK gamma/NEMO) knockout HEK293T cell lysate (ab257153)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: IKBKG knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab188569 observed at 48 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab188569 Anti-IKK gamma/NEMO antibody [EPR14660] was shown to specifically react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266674 (knockout cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab188569 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human IKBKG (IKK gamma/NEMO) knockout HEK293T cell lysate (ab257153)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: IKBKG knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab178872 observed at 48 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab178872 Anti-IKK gamma/NEMO antibody [EPR16629] was shown to specifically react with IKK gamma/NEMO in wild-type HEK-293T cells in western blot. The band observed in the knockout cell line ab266674 (knockout cell lysate ab257153) lane below 48kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and IKK gamma/NEMO knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab178872 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human IKBKG knockout HEK293T cell lysate (ab257153)Allele-1: 13 bp deletion in exon 2
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Sanger Sequencing - Human IKBKG knockout HEK293T cell lysate (ab257153)Allele-2: 1 bp insertion in exon 2