Human IgD ELISA Kit (ab157708)
Key features and details
- Sensitivity: 4.4545 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Other biological fluids, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IgD ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Other biological fluids -
Assay type
Sandwich (quantitative) -
Sensitivity
4.4545 ng/ml -
Range
6.25 ng/ml - 400 ng/ml -
Recovery
> 85 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IgD Human ELISA kit is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgD in Human samples.
In this assay the IgD present in samples reacts with the anti-IgD antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgD antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound IgD. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgD in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgD in the test sample. The quantity of IgD in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-conjugated anti-Human IgD antibody 1 x 150µl 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml Human IgD Calibrator (lyophilized) 1 vial Human IgD ELISA Microplate 1 unit Stop Solution 1 x 12ml -
Research areas
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Relevance
Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker. -
Alternative names
- IGHD
- Immunoglobulin heavy constant delta
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Database links
- Entrez Gene: 3495 Human
- Omim: 147170 Human
- SwissProt: P01880 Human
Images
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Standard CurveRepresentative standard curve using ab157708 IgD Human ELISA Kit.
