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Human GRN (Granulin) knockout HEK293T cell pellet (ab278941)

Overview

  • Product name

    Human GRN (Granulin) knockout HEK293T cell pellet
    See all Granulin kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 2 bp deletion in exon2.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling.
    Granulin-4 promotes proliferation of the epithelial cell line A431 in culture while granulin-3 acts as an antagonist to granulin-4, inhibiting the growth.

  • Tissue specificity

    In myelogenous leukemic cell lines of promonocytic, promyelocytic, and proerythroid lineage, in fibroblasts, and very strongly in epithelial cell lines. Present in inflammatory cells and bone marrow. Highest levels in kidney.

  • Involvement in disease

    Defects in GRN are the cause of ubiquitin-positive frontotemporal dementia (UP-FTD) [MIM:607485]; also known as tau-negative frontotemporal dementia linked to chromosome 17. Frontotemporal dementia (FTD) is the second most common cause of dementia in people under the age of 65 years. It is an autosomal dominant neurodegenerative disease.

  • Sequence similarities

    Belongs to the granulin family.

  • Post-translational
    modifications

    Granulins are disulfide bridged.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • Acrogranin
    • CLN11
    • GEP
    • GP88
    • Granulin A
    • Granulin B
    • Granulin C
    • Granulin D
    • Granulin E
    • Granulin epithelin
    • Granulin F
    • Granulin G
    • Granulin-7
    • Granulins
    • GRN
    • GRN_HUMAN
    • PC cell derived growth factor
    • PCDGF
    • PEPI
    • PGRN
    • Proepithelin
    • Progranulin
    see all

Properties

Images

  • Western blot - Human GRN (Granulin) knockout HEK293T cell pellet (ab278941)
    Western blot - Human GRN (Granulin) knockout HEK293T cell pellet (ab278941)

    Lane 1:Wild-type HEK293T cell lysate (20 ug)
    Lane 2:GRN knockout HEK293T cell lysate (20 ug)
    Lane 3:HepG2 cell lysate (20 ug)
    Lane 4:HAP1cell lysate (20 ug)

    ab208777 was shown to specifically react with Granulin in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266738 (knockout cell lysate ab257235) was used. Wild-type and Granulin knockout samples were subjected to SDS-PAGE. ab208777 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human GRN (Granulin) knockout HEK293T cell pellet (ab278941)
    Sanger Sequencing - Human GRN (Granulin) knockout HEK293T cell pellet (ab278941)

    Allele-1: 14 bp deletion in exon2

     

  • Sanger Sequencing - Human GRN (Granulin) knockout HEK293T cell pellet (ab278941)
    Sanger Sequencing - Human GRN (Granulin) knockout HEK293T cell pellet (ab278941)

    Allele-2: 2 bp deletion in exon2

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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