Human GDNF ELISA Kit (Glial Derived Neurotrophic Factor) (ab100525)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human GDNF (lyophilized) 2 vials GDNF Microplate (12 x 8 wells) 1 unit Recombinant Human GDNF Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Glial cell line derived neurotrophic factor (GDNF) is a highly conserved neurotrophic factor that is a distant member of the TGF beta superfamily. The GDNF gene product is processed to a disulphide-linked homodimer, which is a ligand for the RET protooncogene. GDNF protects and repairs dopamine-containing neurons, which degenerate in Parkinson's disease, and motor neurons, which die in amyotrophic lateral sclerosis. The use of GDNF in the treatment of Parkinson's disease has shown promise in the clinic. Treatment of spinal cord injuries with GDNF has also produced neurological improvement. -
Cellular localization
Secreted -
Alternative names
- Astrocyte derived trophic factor
- ATF
- ATF1
see all -
Database links
- Entrez Gene: 2668 Human
- Omim: 600837 Human
- SwissProt: P39905 Human
- Unigene: 248114 Human
Images
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Typical standard curve
Representative standard curve using ab100525
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ab100525 GDNF (Glial Derived Neurotrophic Factor) Human ELISA KitHuGDNF measured in biological fluids showing quantity (pg) per mL of tested sample
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Typical standard curveRepresentative standard curve using ab100525