Human FOXO3A ELISA Kit (ab277471)
Key features and details
- Sensitivity: 0.14 ng/ml
- Range: 0.137 ng/ml - 10 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FOXO3A ELISA Kit
See all FOXO3A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.14 ng/ml -
Range
0.137 ng/ml - 10 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 132.4 111% - 144% Plasma 108.5 70% - 145% Cell culture media 132.3 120% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human FOXO3A ELISA Kit (ab277471) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FOXO3A in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human FOXO3A coated on a 96-well plate. Standards and samples are pipetted into the wells and Human FOXO3A present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human FOXO3A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human FOXO3A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml 700X HRP-Streptavidin Concentrate 1 x 200µl Biotinylated Anti-Human FOXO3A Antibody 2 vials Anti-Human FOXO3A coated Microplate 1 unit Human FOXO3A standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. -
Tissue specificity
Ubiquitous. -
Involvement in disease
Note=A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. -
Sequence similarities
Contains 1 fork-head DNA-binding domain. -
Post-translational
modificationsIn the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Phosphorylated by PIM1. -
Cellular localization
Cytoplasm > cytosol. Nucleus. Translocates to the nucleus upon oxidative stress and in the absence of survival factors. - Information by UniProt
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Alternative names
- AF6q21
- AF6q21 protein
- DKFZp781A0677
see all -
Database links
- Entrez Gene: 2309 Human
- Omim: 602681 Human
- SwissProt: O43524 Human
- Unigene: 220950 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.
