Anti-PCNA antibody (ab15497)
Key features and details
- Rabbit polyclonal to PCNA
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PCNA antibody
See all PCNA primary antibodies -
Description
Rabbit polyclonal to PCNA -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant full length protein corresponding to Human PCNA.
Database link: P12004 -
General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PCNA antibody (ab15497)All lanes : Anti-PCNA antibody (ab15497) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : IRDye 680 conjugated goat anti-rabbit IgG (H&L) at 1/15000 dilution
Predicted band size: 29 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody (ab15497)ab15497 staining PCNA in Human tonsil tissue by Immunohistochemisty (Formalin/PFA-fixed paraffin-embedded sections).
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Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody (ab15497)ICC/IF image of ab15497 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum ab7481 / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15497, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG(H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-PCNA antibody (ab15497)Anti-PCNA antibody (ab15497) at 1/25 dilution + Raji cell lysate
Predicted band size: 29 kDa
Observed band size: 29 kDa
