Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 29 pg/ml
- Range: 125 pg/ml - 8000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Fibrinogen ELISA Kit - high sensitivity
See all Fibrinogen kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 3.4% Inter-assay Sample n Mean SD CV% serum 3 11.7% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
29 pg/ml -
Range
125 pg/ml - 8000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 94 93% - 95% Saliva 101 98% - 104% Milk 103 102% - 105% Urine 100 99% - 100% Serum 91 90% - 94% Hep Plasma 95 95% - % EDTA Plasma 93 91% - 94% Cit plasma 94 91% - 96% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human Fibrinogen ELISA Kit - high sensitivity (ab241383) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Fibrinogen protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Fibrinogen with 29 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
ASSAY SPECIFICITY
This kit recognizes both native and recombinant human Fibrinogen protein in serum, plasma, urine, saliva, milk, and cell culture supernatant only.
Cell and tissue extract samples have not been tested with this kit.
CROSS REACTIVITY
The following recombinant proteins were prepared at 50 ng/mL and 8,000 pg/mL and assayed for cross reactivity and no cross reactivity was observed: Human Fibrinogen alpha, Human Fibrinogen beta, Human Fibrinogen gamma, Human Factor XII, Human Plasmin, Human Thrombin, Rat Fibrinogen
The following recombinant proteins were prepared at 50 ng/mL and 8,000 pg/mL and assayed for cross reactivity.
- Human D-Dimer: 27% cross-reactivity
SPECIES REACTIVITY
This kit recognizes human Fibrinogen protein.
Native purified mouse Fibrinogen was prepared at 8,000 pg/mL and assayed for cross reactivity. 100% cross reactivity was observed.
Other species reactivity was determined by measuring 1:670 diluted serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Fibrinogen Capture Antibody 1 x 600µl 10X Human Fibrinogen Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Human Fibrinogen Lyophilized Purified Protein (ab84410) 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in FGA are a cause of congenital afibrinogenemia (CAFBN) [MIM:202400]. This is a rare autosomal recessive disorder characterized by bleeding that varies from mild to severe and by complete absence or extremely low levels of plasma and platelet fibrinogen. Note=The majority of cases of afibrinogenemia are due to truncating mutations. Variations in position Arg-35 (the site of cleavage of fibrinopeptide a by thrombin) leads to alpha-dysfibrinogenemias.
Defects in FGA are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Domain
A long coiled coil structure formed by 3 polypeptide chains connects the central nodule to the C-terminal domains (distal nodules). The long C-terminal ends of the alpha chains fold back, contributing a fourth strand to the coiled coil structure. -
Post-translational
modificationsThe alpha chain is not glycosylated.
Forms F13A-mediated cross-links between a glutamine and the epsilon-amino group of a lysine residue, forming fibronectin-fibrinogen heteropolymers.
About one-third of the alpha chains in the molecules in blood were found to be phosphorylated.
Conversion of fibrinogen to fibrin is triggered by thrombin, which cleaves fibrinopeptides A and B from alpha and beta chains, and thus exposes the N-terminal polymerization sites responsible for the formation of the soft clot. The soft clot is converted into the hard clot by factor XIIIA which catalyzes the epsilon-(gamma-glutamyl)lysine cross-linking between gamma chains (stronger) and between alpha chains (weaker) of different monomers.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- FGA
- FGB
- FGG
see all -
Database links
- Entrez Gene: 2244 Human
- Entrez Gene: 2266 Human
- Entrez Gene: 2243 Human
- Omim: 134820 Human
- Omim: 134850 Human
- Omim: 134830 Human
- SwissProt: P02671 Human
- SwissProt: P02675 Human
see all
Images
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Other - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Human Fibrinogen Standard Curve ComparisonStandard Curve comparison between human Fibrinogen current (new) SimpleStep ELISA kit and original ELISA kit (ab208036). The current SimpleStep ELISA kit shows increased sensitivity.
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Example of human Fibrinogen standard curve in Sample Diluent NS.The Fibrinogen standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Example of human Fibrinogen standard curve in Sample Diluent NS.The Fibrinogen standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Fibrinogen in human serum and plasma samples.The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:670, plasma (citrate) 1:5x105, plasma (EDTA) 1:5x105, and plasma (heparin) 1:5x105. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 3.45 µg/mL in serum, 2.86 mg/mL in plasma (citrate), 2.96 mg/mL in plasma (EDTA), and 2.64 mg/mL in plasma (heparin).
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Interpolated concentrations of native Fibrinogen in human urine, saliva, milk and cell culture supernatant samples.The concentrations of Fibrinogen were measured in duplicates, interpolated from the Fibrinogen standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 1:1.7, saliva 1:133, milk 1:133 and HepG2 supernatant 1:500. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 5.93 ng/mL in urine, 934.5 ng/mL in saliva, 199.7 ng/mL in milk and 1.35 µg/mL in HepG2 supernatant.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Fibrinogen concentration was determined to be 1,070 ng/mL with a range of 647 – 2,027 ng/mL.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Linearity of dilution.Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.
Native Fibrinogen was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.
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Sandwich ELISA - Human Fibrinogen ELISA Kit - high sensitivity (ab241383)To learn more about the advantages of recombinant antibodies see here.
