Human Factor XI ELISA Kit (with plasma controls) (ab168547)
Key features and details
- Sensitivity: 1.3 ng/ml
- Range: 1.562 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Factor XI ELISA Kit (with plasma controls)
See all FXI kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5% Inter-assay Sample n Mean SD CV% Overall 8.7% -
Sample type
Cell culture supernatant, Serum, Plasma, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
> 1.3 ng/ml -
Range
1.562 ng/ml - 50 ng/ml -
Recovery
= 95 %
Sample specific recovery Sample type Average % Range Serum 93% - 101% Plasma 91% - 103% -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Factor XI Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Factor XI concentrations in cell culture supernatants, serum and plasma.
A Factor XI specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Factor XI specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Factor XI captured in plate.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent M Concentrate 1 x 30ml 20X Wash Buffer Concentrate 2 x 30ml 50X Biotinylated Human Factor XI Antibody 1 x 120µl Chromogen Substrate 1 x 7ml Factor XI Microplate (12 x 8 well strips) 1 unit Factor XI Standard 1 vial Negative control (Depleted Human Plasma) 1 vial Positive control (Reference Plasma Control) 1 vial Sealing Tapes 3 units Stop Solution 1 x 11ml -
Research areas
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Function
Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX. -
Tissue specificity
Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells. -
Involvement in disease
Defects in F11 are the cause of factor XI deficiency (FA11D) [MIM:612416]; also known as plasma thromboplastin antecedent deficiency or Rosenthal syndrome. It is a hemorrhagic disease characterized by reduced levels and activity of factor XI resulting in moderate bleeding symptoms, usually occurring after trauma or surgery. Patients usually do not present spontaneous bleeding but women can present with menorrhagia. Hemorrhages are usually moderate. -
Sequence similarities
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
Contains 4 apple domains.
Contains 1 peptidase S1 domain. -
Post-translational
modificationsActivated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Coagulation factor XI
- Coagulation factor XIa light chain
- F11
see all -
Database links
- Entrez Gene: 2160 Human
- Omim: 264900 Human
- SwissProt: P03951 Human
- Unigene: 1430 Human
Images
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Typical Standard Curve
Representative Standard Curve using ab168547
