Human FABP2 ELISA Kit (ab193700)
Key features and details
- Sensitivity: 25 pg/ml
- Range: 25 pg/ml - 100000 pg/ml
- Sample type: Cell culture media, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich
- Reacts with: Human
Overview
-
Product name
Human FABP2 ELISA Kit
See all I-FABP kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Serum, Plasma, Cell culture media -
Assay type
Sandwich -
Sensitivity
25 pg/ml -
Range
25 pg/ml - 100000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 129.8 120% - 136% Plasma 126.5 110% - 135% Cell culture media 112.4 103% - 123% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's FABP2 Human ELISA Kit (ab193700) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human FABP2 in sera, plasma and cell culture supernatants.
This assay employs an antibody specific for human FABP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures FABP2 present in the samples. The wells are washed and biotinylated anti-human FABP2 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of FABP2 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.
-
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 1500X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated Human FABP2 detection antibody (lyophilized) 2 vials Human FABP2 Standards (lyophilized) 2 vials Pre-coated Human FABP2 Microplate (12 strips x 8 wells) 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor. -
Tissue specificity
Expressed in the small intestine and at much lower levels in the large intestine. Highest expression levels in the jejunum. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Alternative names
- FABP 2
- FABP2
- FABPI
see all -
Database links
- Entrez Gene: 2169 Human
- Omim: 134640 Human
- SwissProt: P12104 Human
- Unigene: 282265 Human
Images
-
Example Standard Curve
