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Anti-H-FABP antibody [67D3] (ab16916)

Key features and details

  • Mouse monoclonal [67D3] to H-FABP
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-H-FABP antibody [67D3]
    See all H-FABP primary antibodies

  • Description

    Mouse monoclonal [67D3] to H-FABP

  • Host species

    Mouse

  • Specificity

    Ab16916 binds to human H-FABP. It shows no binding to human intestinal or liver FABP and slightly cross reacts with B-FABP.

  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Full length native protein (purified) corresponding to Human H-FABP.

  • Positive control

    • Heart cells, recombinant human H-FABP This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.

  • General notes


    This antibody can be used to detect ischemia areas of human heart. It is also useful as marker for brain damage.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-H-FABP antibody [67D3] (ab16916)
    Immunohistochemistry (Frozen sections) - Anti-H-FABP antibody [67D3] (ab16916)

    Immunohistochemical staining of H-FABP in human skeletal muscle tissue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [67D3] (ab16916)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-H-FABP antibody [67D3] (ab16916)

    IHC image of H-FABP staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16916, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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