Anti-H-FABP antibody (ab45966)
Key features and details
- Rabbit polyclonal to H-FABP
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-H-FABP antibody
See all H-FABP primary antibodies -
Description
Rabbit polyclonal to H-FABP -
Host species
Rabbit -
Specificity
ab45966 reacts with H-FABP, but does not react with liver or intestinal forms of this protein.
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Tested Applications & Species
See all applications and species dataApplication Species WB HumanRecombinant fragment
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Immunogen
Synthetic peptide corresponding to Human H-FABP aa 100 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab46185)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanRecombinant fragmentAll applications MouseRatCowPigApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).Target
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Function
FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 281758 Cow
- Entrez Gene: 2170 Human
- Entrez Gene: 14077 Mouse
- Entrez Gene: 399532 Pig
- Entrez Gene: 79131 Rat
- Omim: 134651 Human
- SwissProt: P10790 Cow
- SwissProt: P05413 Human
see all -
Alternative names
- 422 protein antibody
- Cardiac FABP antibody
- Cardiac Fatty Acid Binding Protein antibody
see all
Images
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Western blot - Anti-H-FABP antibody (ab45966)All lanes : Anti-H-FABP antibody (ab45966) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Ab45966 does not cross-react with small intestine or liver FABP. -
Western blot - Anti-H-FABP antibody (ab45966)Ab45966 recognizes the full length tagged recombinant protein (ab109160) which has an expected molecular weight of 16 kDa.
Datasheets and documents
References (10)
ab45966 has been referenced in 10 publications.
- Chassen SS et al. Placental fatty acid transport across late gestation in a baboon model of intrauterine growth restriction. J Physiol 598:2469-2489 (2020). PubMed: 32338384
- Baati N et al. New evidence of exercise training benefits in myostatin-deficient mice: Effect on lipidomic abnormalities. Biochem Biophys Res Commun 516:89-95 (2019). PubMed: 31200956
- Shrestha N et al. Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells. Cell Physiol Biochem 52:94-108 (2019). PubMed: 30790507
- Šrámek J et al. Hypoxia Modulates Effects of Fatty Acids on NES2Y Human Pancreatic ß-cells. Int J Mol Sci 20:N/A (2019). PubMed: 31336948
- Baati N et al. Myostatin deficiency is associated with lipidomic abnormalities in skeletal muscles. Biochim Biophys Acta 1862:1044-1055 (2017). WB ; Mouse . PubMed: 28676454
- Del Collado M et al. Fatty Acid Binding Protein 3 And Transzonal Projections Are Involved In Lipid Accumulation During In Vitro Maturation Of Bovine Oocytes. Sci Rep 7:2645 (2017). WB . PubMed: 28572619
- Li S et al. Betaine affects muscle lipid metabolism via regulating the fatty acid uptake and oxidation in finishing pig. J Anim Sci Biotechnol 8:72 (2017). WB ; Pig . PubMed: 28883917
- Banu SK et al. Editor's Highlight: Exposure to CrVI during Early Pregnancy Increases Oxidative Stress and Disrupts the Expression of Antioxidant Proteins in Placental Compartments. Toxicol Sci 155:497-511 (2017). PubMed: 28077780
- Tsukahara R et al. Heart-type fatty-acid-binding protein (FABP3) is a lysophosphatidic acid-binding protein in human coronary artery endothelial cells. FEBS Open Bio 4:947-51 (2014). PubMed: 25426414
- Antohe F et al. Heart microvessels and aortic endothelial cells express the 15 kDa heart-type fatty acid-binding proteins. Eur J Cell Biol 76:102-9 (1998). IHC-P ; Rat . PubMed: 9696349
Images
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Western blot - Anti-H-FABP antibody (ab45966)All lanes : Anti-H-FABP antibody (ab45966) at 1 µg/ml
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 4 : Human small intestine tissue lysate - total protein (ab29276)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit IgG secondary antibody (ab28446) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Ab45966 does not cross-react with small intestine or liver FABP. -
Western blot - Anti-H-FABP antibody (ab45966)Ab45966 recognizes the full length tagged recombinant protein (ab109160) which has an expected molecular weight of 16 kDa.
