Human Glucagon ELISA Kit (ab267567)
Key features and details
- Sensitivity: 2.5 pg/ml
- Range: 2.03 pg/ml - 130 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Human Glucagon ELISA Kit
See all Glucagon kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (qualitative) -
Sensitivity
2.5 pg/ml -
Range
2.03 pg/ml - 130 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 89.19 73% - 104% Plasma 86.4 74% - 99% Cell culture media 97.2 88% - 106% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Glucagon ELISA Kit is designed for the quantitative determination of Glucagon in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human Glucagon coated on a 96-well plate. Standards and samples are pipetted into the wells and Glucagon present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Glucagon antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Glucagon bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Human Glucagon coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Human Glucagon Detection Antibody 2 vials Human Glucagon Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
Glucagon is a hormone that is secreted by alpha cells in the pancreas. Glucagon antagonizes insulin by converting glycogen to glucose in the liver and increasing blood sugar levels. Glucagon-like peptide 1 (GLP1), Glucagon-like peptide 2 (GLP2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are in the glucagons hormone family. GLP1 is a transmitter in the central nervous system that regulates feeding and drinking behavior. GLP2 stimulates intestinal epithelial growth. -
Cellular localization
Secreted -
Alternative names
- GCG
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Database links
- Entrez Gene: 2641 Human
- Omim: 138030 Human
- SwissProt: P01275 Human
- Unigene: 516494 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.