Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human FABP2 ELISA Kit (ab234566)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.7 pg/ml
  • Range: 12.5 pg/ml - 800 pg/ml
  • Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human FABP2 ELISA Kit
    See all I-FABP kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 9 1.8%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 3.3%

  • Sample type

    Cell culture supernatant, Urine, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.7 pg/ml

  • Range

    12.5 pg/ml - 800 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 83 82% - 85%
    Serum 119 111% - 125%
    Cell culture extracts 95 92% - 97%
    Cell culture media 100 98% - 102%
    Hep Plasma 108 103% - 110%
    EDTA Plasma 115 109% - 120%
    Cit plasma 113 105% - 118%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human FABP2 ELISA Kit (ab234566) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of FABP2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human FABP2 with 1.7 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human FABP2 ELISA Kit (ab234566)
    Other - Human FABP2 ELISA Kit (ab234566)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human FABP2 standard curve in Sample Diluent NS.
    Example of human FABP2 standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human FABP2 standard curve in 1X Cell Extraction Buffer PTR.
    Example of human FABP2 standard curve in 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native FABP2 in human serum, plasma, and LoVo cell extract samples.
    Interpolated concentrations of native FABP2 in human serum, plasma, and LoVo cell extract samples.

    The concentrations of FABP2 were measured in duplicates, interpolated from the FABP2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (heparin) 50%, plasma (EDTA) 25%, plasma (citrate) 50%, and LoVo extract 750 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration in the neat samples was determined to be 1,248 pg/mL in serum, 1,104 pg/mL in plasma (heparin), 1,642 pg/mL in plasma (EDTA), and 1,199 pg/mL in plasma (citrate).

  • Interpolated concentrations of spiked FABP2 in human urine and RPMI media with 10% FBS.
    Interpolated concentrations of spiked FABP2 in human urine and RPMI media with 10% FBS.

    The concentrations of FABP2 were measured in duplicate and interpolated from the FABP2 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean FABP2 concentration was determined to be 1,094 pg/mL with a range of 422 – 3,639 pg/mL.

  • Human FABP2 Competitor Std Curve Comparison
    Human FABP2 Competitor Std Curve Comparison

    Standard curve comparison between human FABP2 SimpleStep ELISA® kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

  • Sandwich ELISA - Human FABP2 ELISA Kit (ab234566)
    Sandwich ELISA - Human FABP2 ELISA Kit (ab234566)
    To learn more about the advantages of recombinant antibodies see here.

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