Human EIF2AK4 (GCN2) knockout HEK293T cell pellet (ab279233)
Overview
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Product name
Human EIF2AK4 (GCN2) knockout HEK293T cell pellet
See all GCN2 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HEK293T -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon9 and 1 bp insertion in exon9. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human EIF2AK4 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
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Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Target
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Function
Can phosphorylate the alpha subunit of EIF2 and may mediate translational control. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
Contains 2 protein kinase domains.
Contains 1 RWD domain. -
Domain
Kinase domain 1 is a degenerate kinase domain.
RWD domain is reported to interact with GCN1L1. -
Post-translational
modificationsAutophosphorylated on threonine residues. - Information by UniProt
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Alternative names
- E2AK4_HUMAN
- Eif2ak4
- Eukaryotic Translation Initiation Factor 2 alpha kinase 4
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human EIF2AK4 knockout HEK293T cell pellet 1 vial Human wild-type HEK293T cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Images
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Western blot - Human EIF2AK4 (GCN2) knockout HEK293T cell pellet (ab279233)Lane 1: Wild-type HEK-293T cell lysate (20µg)
Lane 2: EIF2AK4 knockout HEK-293T cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab134053 observed at 187 kDa. Red - loading control ab7291 observed at 50 kDa.
ab134053 Anti-GCN2 antibody [EPR5970(2)] was shown to specifically react with GCN2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab267246 (knockout cell lysate ab256902) was used. Wild-type and GCN2 knockout samples were subjected to SDS-PAGE. ab134053 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human EIF2AK4 (GCN2) knockout HEK293T cell pellet (ab279233)Allele-1: 16 bp deletion in exon9
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Sanger Sequencing - Human EIF2AK4 (GCN2) knockout HEK293T cell pellet (ab279233)Allele-2: 1 bp insertion in exon9
