Anti-eIF4E antibody [5D11] (ab171091)
![Anti-eIF4E antibody [5D11] (ab171091)](https://www.abcam.com/ps/products/171/ab171091/Images/ab171091-171583-anti-eif4e-antibody-5d11-western-blot.jpg "Anti-eIF4E antibody [5D11] (ab171091)")
Key features and details
- Mouse monoclonal [5D11] to eIF4E
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-eIF4E antibody [5D11]
See all eIF4E primary antibodies -
Description
Mouse monoclonal [5D11] to eIF4E -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIP HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human eIF4E. Produced in HEK293T cells.
Database link: P06730 -
Positive control
- Hela, MCF7, K562, Jurkat, U2OS and HepG2 lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 0.1% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
5D11 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-eIF4E antibody [5D11] (ab171091)All lanes : Anti-eIF4E antibody [5D11] (ab171091) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : U2OS whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : C2C12 whole cell lysate
Lane 8 : NIH3T3 whole cell lysate
Lane 9 : NRK whole cell lysate
Lysates/proteins at 80 µg per lane.
Secondary
All lanes : Goat anti-mouse-HRP at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
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![Immunoprecipitation - Anti-eIF4E antibody [5D11] (ab171091)](https://www.abcam.com/ps/products/171/ab171091/Images/ab171091-171581-anti-eif4e-antibody-5d11-immunoprecipitation.jpg)
Immunoprecipitation - Anti-eIF4E antibody [5D11] (ab171091) -
![Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody [5D11] (ab171091)](https://www.abcam.com/ps/products/171/ab171091/Images/ab171091-201004-anti-eif4e-antibody-5d11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody [5D11] (ab171091)ab171091 staining eIF4E (green) in the cytoplasm of MCF-7 cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
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![Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody [5D11] (ab171091)](https://www.abcam.com/ps/products/171/ab171091/Images/ab171091-201003-anti-eif4e-antibody-5d11-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-eIF4E antibody [5D11] (ab171091)ab171091 staining eIF4E (green) in the cytoplasm of Hela cells (right) compared with a negative control in the absence of primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes, blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight 488-conjugated goat-anti-mouse IgG (H+L) secondary antibody was used as the secondary antibody. Red (phalloidin) - F-actin, Blue (DAPI) - nuclei. Images were taken at a magnification of 60x.
