Human DPP2 ELISA Kit (DPP7) (ab267618)
Key features and details
- Sensitivity: 0.11 ng/ml
- Range: 0.102 ng/ml - 25 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DPP2 ELISA Kit (DPP7) -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.11 ng/ml -
Range
0.102 ng/ml - 25 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101.1 91% - 112% Plasma 90.53 75% - 117% Cell culture media 92.39 81% - 108% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human DPP2 ELISA Kit (DPP7) is designed for the quantitative determination of DPP2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for human DPP2 coated on a 96-well plate. Standards and samples are pipetted into the wells and DPP2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human DPP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of DPP2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Human DPP2 coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Human DPP2 Detection Antibody 2 vials Human DPP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Relevance
DPP2 is a serine protease found most abundantly in the lysosome. There is also a non-lysosomal membrane-associated form of DPP2 reported in the rat brain (DPP-II-M), and a soluble form (DPP-II-S). First discovered as an enzyme involved in the terminal steps of protein degradation, and later as an enzyme involved in cell growth and quiescence, DPP2 cleaves N-terminal dipeptides and tripeptides from proteins. DPP2 has a preference for proline in the P1 position, and processes the tripeptides generated by DPP1 (which is unable to cleave if proline is in the P1 position). Some tumor cell lines express elevated DPP2 levels, and serum levels of DPP2 are elevated in some cancer patients, making DPP2 a potential tumor marker. DPP2 levels have been also been reported to be elevated in the cerebral spinal fluid from Parkinson’s patients. -
Cellular localization
Lysosome. Cytoplasmic vesicle. Secreted. -
Alternative names
- Dipeptidyl aminopeptidase II
- Dipeptidyl peptidase 7
- Dipeptidyl peptidase II
see all -
Database links
- Entrez Gene: 29952 Human
- Omim: 610537 Human
- SwissProt: Q9UHL4 Human
- Unigene: 37916 Human
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.