Human DLK1 ELISA Kit (ab270211)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 35 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human DLK1 ELISA Kit
See all DLK-1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 3 2.4% Inter-assay Sample n Mean SD CV% Serum 8 4.1% -
Sample type
Serum, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
35 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100 97% - 104% Tissue Extracts 98 91% - 106% Hep Plasma 92 81% - 100% EDTA Plasma 100 97% - 101% Cit plasma 101 97% - 104% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human DLK1 ELISA Kit (ab270211) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DLK1 protein in serum, cit plasma, hep plasma, tissue extracts, and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human DLK1 with 35 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Protein delta homolog 1 (DLK1) is a protein that in humans, is encoded by the DLK1 gene. DLK1 is expressed as a transmembrane protein, but a soluble form cleaved by ADAM17 is active in inhibiting adipogenesis, the differentiation of pre-adipocytes into adipocytes. It is a member of the EGF-like family of homeotic proteins. DLK1 is found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. Human DLK1 shares 85% sequence homology with mouse and rat, 83% and 93% with bovine and monkey, respectively.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human DLK1 Capture Antibody 1 x 600µl 10X Human DLK1 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human DLK1 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May have a role in neuroendocrine differentiation. -
Tissue specificity
Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. -
Sequence similarities
Contains 6 EGF-like domains. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- Adipocyte differentiation inhibitor protein
- Brevideltin
- Delta like 1 homolog
see all -
Database links
- Entrez Gene: 8788 Human
- Omim: 176290 Human
- SwissProt: P80370 Human
- Unigene: 533717 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human DLK1 standard curve in Sample Diluent NS.The DLK1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native DLK1 in human serum and plasma samples.The concentrations of DLK1 were measured in duplicates, interpolated from the DLK1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 12.5%, plasma (citrate) 25%, plasma (EDTA) 25%, and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DLK1 concentration was determined to be 10.36 ng/mL in serum, 8.41 ng/mL in plasma (citrate), 5.99 ng/mL in plasma (EDTA), and 10.26 ng/mL in plasma (heparin).
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Interpolated concentrations of native DLK1 in human placenta extract sample based on an 80 µg/mL extract load.The concentrations of DLK1 were measured in duplicate and interpolated from the DLK1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DLK1 concentration was determined to be 1,087 pg/mL in placenta extract.
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Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DLK1 concentration was determined to be 7 ng/mL with a range of 0 – 13 ng/mL.
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Sandwich ELISA - Human DLK1 ELISA Kit (ab270211)To learn more about the advantages of recombinant antibodies see here.
