Human DDIT4 (REDD-1) knockout HeLa cell pellet (ab279022)
Overview
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Product name
Human DDIT4 (REDD-1) knockout HeLa cell pellet
See all REDD-1/DDIT4 kits -
Product overview
Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.
Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation. -
Parental Cell Line
HeLa -
Organism
Human -
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2. -
Passage number
Knockout validation
Sanger Sequencing, Western Blot (WB)Notes
Pellet size: 5 million cells/vial.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Tested applications
Suitable for: WBmore detailsProperties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human DDIT4 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Target
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Function
Inhibits cell growth by regulating the TOR signaling pathway upstream of the TSC1-TSC2 complex and downstream of AKT1. Promotes neuronal cell death. -
Tissue specificity
Broadly expressed, with lowest levels in brain, skeletal muscle and intestine. Up-regulated in substantia nigra neurons from Parkinson disease patients (at protein level). -
Sequence similarities
Belongs to the DDIT4 family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Alternative names
- DDIT4
- DDIT4_HUMAN
- Dig2
see all
Properties
-
Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human DDIT4 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
-
Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human DDIT4 (REDD-1) knockout HeLa cell pellet (ab279022)
Lane 1:Wild-type HeLa cell lysate (20 ug)
Lane 2:DDIT4 knockout HeLa cell lysate (20 ug)
Lane 3:HeLa treated with 0.5mM CoCL2 for 6 hours cell lysate (20 ug)
Lane 4:Untreated HeLa cell lysate (20 ug)ab191871 Anti-REDD-1/DDIT4 antibody [EPR18716] was shown to specifically react with REDD-1/DDIT4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264834 (knockout cell lysate ab257409) was used. Wild-type and REDD-1/DDIT4 knockout samples were subjected to SDS-PAGE. ab191871 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human DDIT4 (REDD-1) knockout HeLa cell pellet (ab279022)Homozygous: 1 bp insertion in exon 2