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Human Heat Shock Factor Protein 1 ELISA kit (ab222879)

Key features and details

  • Sensitivity: 0.5 ng/ml
  • Range: 0.625 ng/ml - 20 ng/ml
  • Sample type: Cell Lysate, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human, Pig, Monkey

Overview

  • Product name

    Human Heat Shock Factor Protein 1 ELISA kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 4.9%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 9.8%

  • Sample type

    Tissue Extracts, Cell Lysate

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    0.5 ng/ml

  • Range

    0.625 ng/ml - 20 ng/ml

  • Assay time

    4h 00m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human, Pig, Monkey
    Does not react with: Mouse, Rabbit

  • Product overview

    The Human HSF 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222879) is designed for detection of human HSF 1 in cell lysate and tissue extract samples.


    This assay employs a quantitative sandwich enzyme immunoassay technique that measures human HSF 1 in approximately 4 hours. A polyclonal antibody specific for human HSF 1 has been pre-coated onto a 96-well microplate with removable strips. HSF 1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human HSF 1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Notes

    Heat shock factor protein 1 (HSF 1), also known as heat shock transcription factor 1 or HSTF 1, is a 529-amino acid transcription factor of 57 kDa. HSF 1 is present in unstressed cells as an inactive monomeric form and becomes activated by heat and other stress stimuli. HSF 1 is rapidly induced after temperature stress and binds heat shock promoter elements (HSE) that are present upstream of all the heat shock genes. Heat shock protein 90 (HSP90) inhibits HSF 1 activation. HSP90-containing HSF 1 complex is present in the unstressed cell and dissociates during stress. HSF 1 plays a crucial role in inducing heat shock proteins, which is required for thermotolerance. It is involved in oogenesis, spermatogenesis, placental development, and regulation of lifespan.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    1X Standard Diluent 1 x 2ml
    20X Wash Buffer Concentrate 2 x 30ml
    40X Biotinylated Human HSF 1 1 x 150µl
    Anti- Human HSF 1 coated Microplate (12 x 8 wells) 1 unit
    Chromogen Substrate 1 x 8ml
    Human HSF 1 Standard 1 vial
    Sealing Tapes 1 x 3 units
    Stop Solution 1 x 12ml

  • Research areas

  • Function

    DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked.

  • Sequence similarities

    Belongs to the HSF family.

  • Domain

    the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors.

  • Post-translational
    modifications

    Phosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
    Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation.

  • Cellular localization

    Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules.
  • Information by UniProt

  • Alternative names

    • Heat shock factor 1
    • Heat shock factor protein 1
    • Heat shock transcription factor 1
    • HSF 1
    • hsf1
    • HSF1_HUMAN
    • HSTF 1
    • HSTF1
    see all

  • Database links

    Images

    • Human HSF 1 standard curve
      Human HSF 1 standard curve

      Example of human HSF 1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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