Human Heat Shock Factor Protein 1 ELISA kit (ab222879)
Key features and details
- Sensitivity: 0.5 ng/ml
- Range: 0.625 ng/ml - 20 ng/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human, Pig, Monkey
Overview
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Product name
Human Heat Shock Factor Protein 1 ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Plasma 4.9% Inter-assay Sample n Mean SD CV% Plasma 9.8% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
0.5 ng/ml -
Range
0.625 ng/ml - 20 ng/ml -
Assay time
4h 00m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human, Pig, Monkey
Does not react with: Mouse, Rabbit -
Product overview
The Human HSF 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab222879) is designed for detection of human HSF 1 in cell lysate and tissue extract samples.
This assay employs a quantitative sandwich enzyme immunoassay technique that measures human HSF 1 in approximately 4 hours. A polyclonal antibody specific for human HSF 1 has been pre-coated onto a 96-well microplate with removable strips. HSF 1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human HSF 1, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Heat shock factor protein 1 (HSF 1), also known as heat shock transcription factor 1 or HSTF 1, is a 529-amino acid transcription factor of 57 kDa. HSF 1 is present in unstressed cells as an inactive monomeric form and becomes activated by heat and other stress stimuli. HSF 1 is rapidly induced after temperature stress and binds heat shock promoter elements (HSE) that are present upstream of all the heat shock genes. Heat shock protein 90 (HSP90) inhibits HSF 1 activation. HSP90-containing HSF 1 complex is present in the unstressed cell and dissociates during stress. HSF 1 plays a crucial role in inducing heat shock proteins, which is required for thermotolerance. It is involved in oogenesis, spermatogenesis, placental development, and regulation of lifespan.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X Streptavidin-Peroxidase Conjugate 1 x 80µl 10X Diluent N Concentrate 1 x 30ml 1X Standard Diluent 1 x 2ml 20X Wash Buffer Concentrate 2 x 30ml 40X Biotinylated Human HSF 1 1 x 150µl Anti- Human HSF 1 coated Microplate (12 x 8 wells) 1 unit Chromogen Substrate 1 x 8ml Human HSF 1 Standard 1 vial Sealing Tapes 1 x 3 units Stop Solution 1 x 12ml -
Research areas
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Function
DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. -
Sequence similarities
Belongs to the HSF family. -
Domain
the 9aaTAD motif is a transactivation domain present in a large number of yeast and animal transcription factors. -
Post-translational
modificationsPhosphorylated on multiple serine residues, a subset of which are involved in stress-related regulation of transcription activation. Constitutive phosphorylation represses transcriptional activity at normal temperatures. Levels increase on specific residues heat-shock and enhance HSF1 transactivation activity. Phosphorylation on Ser-307 derepresses activation on heat-stress and in combination with Ser-303 phosphorylation appears to be involved in recovery after heat-stress. Phosphorylated on Ser-230 by CAMK2, in vitro. Cadmium also enhances phosphorylation at this site. Phosphorylation on Ser-303 is a prerequisite for HSF1 sumoylation. Phosphorylation on Ser-121 inhibits transactivation and promotes HSP90 binding. Phosphorylation on Thr-142 also mediates transcriptional activity induced by heat. Phosphorylation on Ser-326 plays an important role in heat activation of HSF1 transcriptional activity.
Sumoylated with SUMO1 and SUMO2 on heat-shock. Heat-inducible sumoylation occurs after 15 min of heat-shock, after which levels decrease and at 4 hours, levels return to control levels. Sumoylation has no effect on HSE binding nor on transcriptional activity. Phosphorylation on Ser-303 is a prerequisite for sumoylation. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic during normal growth. On activation, translocates to nuclear stress granules. Colocalizes with SUMO1 in nuclear stress granules. - Information by UniProt
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Alternative names
- Heat shock factor 1
- Heat shock factor protein 1
- Heat shock transcription factor 1
see all -
Database links
- Entrez Gene: 3297 Human
- Omim: 140580 Human
- SwissProt: Q00613 Human
- Unigene: 530227 Human