Human Cytokeratin 18 ELISA Kit (ab227896)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 3.4 pg/ml
- Range: 15.6 pg/ml - 1000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cytokeratin 18 ELISA Kit
See all Cytokeratin 18 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 2.1% Inter-assay Sample n Mean SD CV% Overall 3 8.4% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Tissue Extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
3.4 pg/ml -
Range
15.6 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 100 99% - 102% Cell culture extracts 110 106% - 115% Cell culture media 109 107% - 110% Hep Plasma 99 97% - 99% EDTA Plasma 99 97% - 100% Cit plasma 95 93% - 97% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Cytokeratin 18 ELISA Kit (ab227896) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Cytokeratin 18 protein in cell culture extracts, cit plasma, edta plasma, hep plasma, serum, cell culture supernatant, and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Cytokeratin 18 with 3.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml 10X Human Cytokeratin 18 Capture Antibody 1 x 600µl 10X Human Cytokeratin 18 Detector Antibody 1 x 600µl Human Cytokeratin 18 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent LS 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. -
Tissue specificity
Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. -
Involvement in disease
Defects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600]. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Alternative names
- Cell proliferation inducing gene 46 protein
- Cell proliferation inducing protein 46
- Cell proliferation-inducing gene 46 protein
see all -
Database links
- Entrez Gene: 3875 Human
- Omim: 148070 Human
- SwissProt: P05783 Human
- Unigene: 406013 Human
Images
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Other - Human Cytokeratin 18 ELISA Kit (ab227896)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Human Cytokeratin 18 standard curve in Sample Diluent NSBackground-subtracted data values (mean +/- SD) are graphed.
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Example of Human Cytokeratin 18 standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Cytokeratin 18 in Human serum and plasma samplesThe concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 23 pg/mL in serum, 29pg/mL in plasma (EDTA), 96 pg/mL in plasma (heparin), and 33 pg/mL in plasma (citrate).
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Serum from ten individual healthy Human female donors were measured in duplicateInterpolated dilution factor corrected values are plotted (mean +/- SD, n=2). 5/10 donors were below the detectable dose. The mean of the detectable donors Cytokeratin 18 concentration was determined to be 59 pg/mL with a range of 31– 79 pg/mL.
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Interpolated concentrations of spiked Cytokeratin 18 in Human serum and plasma samplesThe concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
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Interpolated concentrations of native Cytokeratin 18 in Human cell culture supernatant sampleThe concentrations of Cytokeratin 18 were measured in duplicates, interpolated from the Cytokeratin 18 standard curves and corrected for sample dilution. Undiluted samples are as follows: Hela cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 1000 pg/mL in Hela cell culture supernatant.
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Interpolated concentrations of native Cytokeratin 18 in Hela cell extract and Human Liver tissue extractThe concentrations of Cytokeratin 18 were measured in duplicate and interpolated from the Cytokeratin 18 standard curve and corrected for sample dilution and extract load. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Cytokeratin 18 concentration was determined to be 0.89 pg / µg Hela extract and 35 pg/ µg Liver extract.
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Sandwich ELISA - Human Cytokeratin 18 ELISA Kit (ab227896)To learn more about the advantages of recombinant antibodies see here.
