Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apc6 antibody [EPR16889] (ab181567)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16889] to Apc6
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Apc6 antibody [EPR16889]
    See all Apc6 primary antibodies

  • Description

    Rabbit monoclonal [EPR16889] to Apc6

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: L-929, HeLa, HepG2, HT1080, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates. Human fetal liver and mouse liver tissue lysate. IP: HeLa

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16889

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Apc6 antibody [EPR16889] (ab181567)
    Western blot - Anti-Apc6 antibody [EPR16889] (ab181567)
    All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilution

    Lane 1 : L-929 (Mouse connective tissue fibroblast cells) whole cell lysates at 20 µg
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates at 20 µg
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysates at 20 µg
    Lane 4 : HT1080 (Human fibrosarcoma cells) whole cell lysates at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Apc6 antibody [EPR16889] (ab181567)
    Western blot - Anti-Apc6 antibody [EPR16889] (ab181567)
    All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/1000 dilution

    Lane 1 : Human fetal liver
    Lane 2 : Mouse liver

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Apc6 antibody [EPR16889] (ab181567)
    Western blot - Anti-Apc6 antibody [EPR16889] (ab181567)
    All lanes : Anti-Apc6 antibody [EPR16889] (ab181567) at 1/5000 dilution

    Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 71 kDa
    Observed band size: 71 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Apc6 antibody [EPR16889] (ab181567)
    Immunoprecipitation - Anti-Apc6 antibody [EPR16889] (ab181567)

    APC6 Protein was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab181567 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181567 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: HeLa whole cell extract. Lane 2: PBS instead of HeLa whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • Anti-Apc6 antibody [EPR16889] (ab181567)
    Anti-Apc6 antibody [EPR16889] (ab181567)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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